农业生物技术学报2025，Vol.33Issue(8)：1755-1766,12.DOI:10.3969/j.issn.1674-7968.2025.08.009
青藏高原黄牛父系遗传多样性研究
Study on Paternal Genetic Diversity of Cattle(Bos taurus)in Qinghai-Tibet Plateau
摘要
Abstract
The Qinghai-Tibet plateau(QTP)cattle(Bos taurus)is a rare local genetic resource of cattle in China plateau region due to its unique geographical environment and numerous breeds.To analyze the paternal genetic diversity and phylogenetic relationship of QTP cattle,this study integrated the whole genome re-sequencing data of 77 male QTP cattle and scanned the single nucleotide polymorphisms(SNPs)and insertions/deletions(INDELs)in the single-copy gene region of the X-degenerate region of the Y chromosome.A total of 2 672 Y-SNPs and 712 Y-INDELs were identified.Based on Y-SNPs,30 Y-SNPs haplotypes of QTP cattle were defined in this study,among which hap 10 was the dominant haplotype,accounting for 16.88％.It was found that QTP cattle belonged to 6 Y haplogroups/sub-haplogroups(Y1,Y2A,Y2B,Y3A2,Y3B1 and Y3B4),among which Y2B(59.74％)was the main haplogroup,individuals of QTP cattle were mainly concentrated in the Y2B haplogroup.The analysis based on Y-INDELs was also consistent with Y-SNPs.The haplotype diversity of Y-SNPs in the QTP cattle population was 0.973±0.006,and the nucleotide diversity was 0.237±0.046.This study confirmed that QTP cattle had 6 paternal origins,including 3 taurine cattle(B.taurus taurus)and 3 indicine cattle(B.taurus indicus)paternal origins,clarified the high paternal genetic diversity of QTP cattle.This study provides a theoretical basis for the protection,development and utilization of QTP cattle.关键词
青藏高原黄牛/Y染色体单核苷酸多态性(Y-SNPs)/Y染色体插入和缺失(Y-INDELs)/单倍型/父系起源Key words
Qinghai-Tibet plateau cattle/Y-SNPs/Y-INDELs/Haplotype/Paternal origin分类
农业科技引用本文复制引用
阎卉萱,雷初朝,巴桑珠扎,陈宁博,姚婷婷,曹一凡,李心怡,次仁罗布,索朗曲吉,尼玛仓决,旦增洛桑,斯朗旺姆..青藏高原黄牛父系遗传多样性研究[J].农业生物技术学报,2025,33(8):1755-1766,12.基金项目
国家自然科学基金(32102523 （）
32260823) （）
西藏自治区重点研究发展计划项目(XZ202301ZY0008N) （XZ202301ZY0008N）
