热带亚热带植物学报2025，Vol.33Issue(4)：372-380,9.DOI:10.11926/jtsb.4942
机械伤害、排胶和割胶频率对橡胶树橡胶生物合成的影响
Effects of Mechanical Wounding,Latex Flow and Tapping Frequency on Rubber Biosynthesis in Hevea brasiliensis
摘要
Abstract
In order to demonstrate the effects of mechanical wounding,latex flow and tapping frequency on rubber biosynthesis in Hevea brasiliensis.In the present study,mature virgin trees were used as test materials.The in vitro rubber biosynthesis efficiency of latex was determined using 13C-MVA as substrate,and the expressions of five key genes of jasmonic acid signaling pathway,HbCOI1,HbJAZ1,HbMYC1,HbMYC2,HbMYC3,and five key protein genes of rubber biosynthesis,HbHMGR1,HbSRPP1,HbREF1,HbHRT1,HbHRT2 in laticifer cells were analyzed by qPCR.The results showed that the promoting effect of tapping on rubber biosynthesis was mainly caused by cumulative latex flow,and low tapping frequency was beneficial to rubber biosynthesis.The results of this study have important theoretical significance for elucidating the regulatory mechanism of natural rubber biosynthesis in laticifer cells,and provide a theoretical basis for designing appropriate rubber tapping frequency in natural rubber production.关键词
巴西橡胶树/割胶/排胶/橡胶生物合成/基因表达Key words
Hevea brasiliensis/Tapping/Latex flow/Rubber biosynthesis/Gene expression引用本文复制引用
杨署光,晁金泉,李言,吴绍华,张世鑫,邓小敏,史敏晶,田维敏..机械伤害、排胶和割胶频率对橡胶树橡胶生物合成的影响[J].热带亚热带植物学报,2025,33(4):372-380,9.基金项目
海南省自然科学基金(321MS0806) （321MS0806）
云南省重大科技计划专项(202402AE09001901) （202402AE09001901）
海南省重点研发计划(ZDYF2022XDNY252) （ZDYF2022XDNY252）
海南省创新团队项目(320CXTD442) （320CXTD442）
国家天然橡胶产业技术体系育种技术与方法项目(CARS-33-YZ1)资助 This work was supported by the Project for Natural Science in Hainan(Grant No.321MS0806),the Project for Major Science and Technology Planning in Yunnan(Grant No.202402AE09001901),the Project for Key Science and Technology Development in Hainan(Grant No.ZDYF2022XDNY252),the Project for Innovation Team in Hainan(Grant No.320CXTD442),and the Project for Breeding Technology and Method of National Natural Rubber Industry Technology System(Grant No.CARS-33-YZ1). （CARS-33-YZ1）
