热带亚热带植物学报2025，Vol.33Issue(4)：399-406,8.DOI:10.11926/jtsb.4933
两步生根法中4种因素对尾巨桉组培苗生根的影响
Effect of Four Factors in Two-step Rooting Method on Rooting of Eucalyptus urophylla×E.grandis Clones
摘要
Abstract
The rooting ability of tissue culture seedlings is directly related to their subsequent transplanting survival rate and growth capacity.The study on the rooting of Eucalyptus urophylla×E.grandis tissue culture seedlings is of great significance for improving the quality of seedling rooting and reducing costs.Four E.urophylla×E.grandis clones(EC262,EC264,EC269 and EC272)from the same hybrid combination were used as experimental materials.A 4-factor(dark culture duration,medium 1,medium 2,clone)4-level orthogonal experiment was designed.The rooting-related indicators were measured 30 days after inoculation,and the data were processed and evaluated by range analysis.The results showed that the rooting rate,number of roots,average root length,rooting trait score rate and callus rate of the clones under different treatments were significantly different at 0.05 level.The rooting rate ranged from 12.5%to 65%;the average number of roots was 1.9 to 3.57;the average root length ranged from 2.55 to 4.81 cm;the rooting trait score rate was 28%to 78%;and the callus rate was 10%to 65%.Range analysis indicated that the medium(containing growth regulators)in the induction stage had the greatest effect on the average root length,with a range of 4.19;the rooting traits were mainly affected by the medium in the root growth stage,with a range of 1.02;and the clone was the most important factor affecting the callus rate,with a range of 8.5.Therefore,the average root length of the four E.urophylla×E.grandis clones during the rooting stage was more affected by the environmental factors in the induction stage,while the rooting traits were more affected by the environmental factors in the growth stage.In addition,although both genetic factors(clones)and environmental factors(dark environment,medium)had significant effects on the callus rate,the genetic factors had a greater influence.关键词
尾巨桉/组培/无性系/生根率/根系形态Key words
Eucalyptus urophylla×E.grandis/Tissue culture/Clone/Rooting rate/Root morphology引用本文复制引用
黄安瀛,罗建中,陈铭秋,卢万鸿,燕青,林彦..两步生根法中4种因素对尾巨桉组培苗生根的影响[J].热带亚热带植物学报,2025,33(4):399-406,8.基金项目
"十四五"国家重点研发计划项目(2022YFD2200203-3) （2022YFD2200203-3）
广东省林业科技创新项目(221KJCX019)资助 This work was supported by the National 14th Five-year Plan Key R&D Program(Grant No.2022YFD2200203-3),and the Project for Forestry Science and Technology Innovation in Guangdong(Grant No.221KJCX019). （221KJCX019）
