热带亚热带植物学报2025，Vol.33Issue(4)：459-466,8.DOI:10.11926/jtsb.4901
白旗兜兰种子共生萌发与非共生萌发的形态解剖学比较研究
Morphological and Anatomical Comparison of Symbiotic and Non-symbiotic Germination of Paphiopedilum spicerianum Seeds
摘要
Abstract
Paphiopedilum spicerianum has high ornamental and conservation value,but its seeds are extremely small and without endosperm,which restricts its mass reproduction.Both symbiotic seed germination and non-symbiotic seed germination techniques can be used for the large-scale production of orchids in vitro,but they have their advantages and disadvantages.The morphological and anatomical changes in symbiotic and non-symbiotic germination of P.spicerianum seeds were compares.The results showed that the process of symbiotic germination was faster than that of non-symbiotic germination,and the growth trend was consistent.The seed germination rate and protocorm differentiation rate under symbiotic germination were higher than those under non-symbiotic germination(VW medium).After sowing for 60 days,the seed germination rate of symbiotic germination reached 92.57%,and the differentiation rate of protocorm reached 65.06%.From paraffin section,the morphogenetic process of symbiotic germination was faster than that of non-symbiotic germination,such as the formation of protocorm,the establishment of stem meristem and the formation of leaf primordium.Under scanning electron microscopy,the polar distribution of starch granules and fungi existed in the corms and seedlings of symbiotic germinations,but not in non-symbiotic germinations.Therefore,symbiotic germination is more advantageous in promoting protocorm morphogenesis under the action of germinating fungi.关键词
白旗兜兰/共生萌发/非共生萌发/形态解剖Key words
Paphiopedilum spicerianum/Symbiotic germination/Non-symbiotic germination/Morphological anatomy引用本文复制引用
李业斐,陈红,李俊泽,曾晶珏,吴坤林,曾宋君,房林..白旗兜兰种子共生萌发与非共生萌发的形态解剖学比较研究[J].热带亚热带植物学报,2025,33(4):459-466,8.基金项目
广州市财政农业农村项目(23106468) （23106468）
广州市科技计划项目(2024B03J1129,2023E04J0004) （2024B03J1129,2023E04J0004）
林芝市科技计划项目(2023-QYCX-02) （2023-QYCX-02）
广东省重点领域研发计划项目(2022B1111040003 （）
2022B1111230001)资助 This work was supported by the Project for Agriculture and Rural of Guangzhou Finance(Grant No.23106468),the Project for Science and Technology in Guangzhou(Grant No.2024B03J1129,2023E04J0004),the Project for Science and Technology in Linzhi(Grant No.2023-QYCX-02),and the Key Technologies R&D Program in Guangdong(Grant No.2022B1111040003,2022B1111230001). （Grant No.23106468）
