肿瘤预防与治疗2025，Vol.38Issue(7)：604-612,9.DOI:10.3969/j.issn.1674-0904.2025.07.007
一种用于头颈部肿瘤药物筛选的微流控芯片
A Microfluidic Chip for Drug Screening in Head and Neck Tumors
摘要
Abstract
Objective:This study designed an automatic microfluidic chip for drug screening of head and neck tumor cells to improve the throughput and rapidity.Methods:Firstly,the design includes minimal chamber volumes for head and neck squamous cell carcinoma(HNSCC)organoid culture,and dual-channel concentration gradient generator.Then,the stability of the chip was verified through simulation and actual operation.Finally,we compared the cell viability inside and outside the chip after drug screening.Results:The concentration gradient based on convection generates six different drug concentra-tions,allowing for stable drug mixing within 100 μL/min.Cell viability of HNSCC was able to decrease with increasing drug concentration during the test,and the results inside and outside the chip were consistent.Conclusion:The study validates the feasibility of microfluidic chip in drug screening on head and neck tumor cells,providing a significant opportunity for the development of personalized treatment plans for head and neck tumors.关键词
头颈部肿瘤/微流控芯片/浓度梯度器/药物筛选Key words
Head and neck tumors/Microfluidic chip/Concentration gradient generator/Drug screening分类
医药卫生引用本文复制引用
张之弥,廖婧如,涂志豪,李灵..一种用于头颈部肿瘤药物筛选的微流控芯片[J].肿瘤预防与治疗,2025,38(7):604-612,9.基金项目
国家自然科学基金(编号:82373242) This study was supported by National Natural Science Foundation of China(No.82373242). （编号:82373242）
