山东农业科学2025，Vol.57Issue(6)：1-14,14.DOI:10.14083/j.issn.1001-4942.2025.06.001
海岛棉R2R3-MYB基因家族的鉴定及其在黄萎病胁迫下的表达分析
Identification of R2R3-MYB Gene Family and Their Expression Analysis under Verticillium Wilt Stress in Gossypium barbadense
摘要
Abstract
R2R3-MYB transcription factors play crucial roles in the process of plants responding to stress.In this study,based on the genomic data of Gossypium barbadense,the members of the R2R3-MYB gene family in G.barbadense were systematically identified.Bioinformatics methods were used to analyze the phyletic evolution,structure,physicochemical properties,and cis-acting elements of the promoter of the R2R3-MYB gene family in G.barbadense.Five candidate genes for Verticillium wilt resistance were screened out using tran-scriptome expression profiles,and the expression patterns of each candidate gene under the induction of Verti-cillium wilt,salicylic acid(SA)and methyl jasmonate(MeJA)were analyzed by real-time fluorescence quan-titative PCR.The results showed that there were 98 R2R3-MYB genes in the genome of G.barbadense,which distributed on 25 chromosomes.The phylogenetic tree analysis indicated that the members of the R2R3-MYB gene family in G.barbadense could be divided into three large subgroups,and they were relatively conserved in evolution.Promoter analysis revealed multiple cis-acting elements related to plant hormones and stress respon-ses.The expression pattern analysis after Verticillium wilt stress showed that all the R2R3-MYB genes in G.barbadense had different degrees of differential expression after Verticillium wilt stress.Five candidate genes for Verticillium wilt resistance in G.barbadense(GbMYB-21,GbMYB-34,GbMYB-39,GbMYB-76,and GbMYB-83)were screened out,which were all induced by Verticillium wilt stress,SA and MeJA.In conclusion,this study demonstrated that the R2R3-MYB gene family in G.barbadense played an important role in cotton disease resistance,which could provide a theoretical basis for further research on the functions of R2R3-MYB genes in G.barbadense and the molecular mechanism of cotton resistance to Verticillium wilt.关键词
海岛棉/R2R3-MYB基因家族/生物信息学分析/黄萎病胁迫/表达分析Key words
Gossypium barbadense/R2R3-MYB gene family/Bioinformatics analysis/Verticillium wilt stress/Expression analysis分类
农业科技引用本文复制引用
胡欣贵,赵杰银,陆佳欣,陈全家,曲延英..海岛棉R2R3-MYB基因家族的鉴定及其在黄萎病胁迫下的表达分析[J].山东农业科学,2025,57(6):1-14,14.基金项目
新疆维吾尔自治区重大科技专项"早熟抗病棉花新品种设计与培育"(2023ZD04041) （2023ZD04041）
新疆"天山英才"培养计划项目(2023TSYCLJ0012) （2023TSYCLJ0012）
