山东农业科学2025，Vol.57Issue(6)：37-46,63,11.DOI:10.14083/j.issn.1001-4942.2025.06.004
黑胫病对大宁晒烟转录组和代谢组的影响
Effects of Black Shank Infection on Transcriptome and Metabolome of Sun-Cured Tobacco in Daning
摘要
Abstract
Daning sun-cured tobacco is a species of good-quality yellow sun-cured tobacco in Hezhou,Guangxi,and it is the excellent raw material for cigarette production.However,it has extremely low resistance to black shank disease.This study investigated the response mechanism of Daning sun-cured tobacco to black shank disease by comparing the differentially expressed genes and metabolites between healthy and black shank-infected leaves through transcriptomic and metabolomic analyses.The results showed that a total of 7 194 significantly differentially expressed genes were identified through transcriptomic analysis,and 198 sig-nificantly different metabolites were detected through metabolomic analysis.The combined transcriptomic and metabolomic analysis revealed that:① Plant hormone signal transduction and phenylpropanoid biosynthesis pathways played important roles in the defense of Daning sun-cured tobacco against black shank infection.②It was speculated that metabolic pathways such as aminoacyl-tRNA biosynthesis,cyanogenic amino acid metabo-lism,valine,leucine and isoleucine degradation,cysteine and methionine metabolism,and arginine and pro-line metabolism were related to the response of Daning sun-cured tobacco to black shank stress.This study elu-cidated the major gene transcriptional regulatory networks and metabolic pathways of Daning sun-cured tobacco in response to black shank infection,which could provide a theoretical basis for research on improving the re-sistance of Daning sun-cured tobacco to black shank disease.关键词
大宁晒烟/黑胫病/转录组/代谢组/植物激素信号转导/苯丙烷类生物合成Key words
Daning sun-cured tobacco/Black shank disease/Transcriptome/Metabolome/Plant hor-mone signal transduction/Phenylpropanoid biosynthesis分类
农业科技引用本文复制引用
祁诗晴,首安发,田梦强,石保峰,杨盟权,刘国顺,殷全玉,余金恒..黑胫病对大宁晒烟转录组和代谢组的影响[J].山东农业科学,2025,57(6):37-46,63,11.基金项目
国家自然科学基金项目(32400218) （32400218）
中国烟草总公司广西壮族自治区公司科技项目(202245000024085,202345100024261) （202245000024085,202345100024261）
河南省科技攻关项目(242102110240) （242102110240）
河南农业大学高层次人才专项支持基金项目(30501474) （30501474）
