上海交通大学学报（医学版）2025，Vol.45Issue(7)：866-873,8.DOI:10.3969/j.issn.1674-8115.2025.07.008
纳米工程化T细胞体系的构建及其对口腔鳞状细胞癌的体外治疗研究
Nanoengineered T cell system for the in vitro treatment of oral squamous cell carcinoma
摘要
Abstract
Objective·To construct a nanoengineered T cell system and explore its synergistic efficacy in the in vitro treatment of oral squamous cell carcinoma,and provide a new strategy for this disease.Methods·Zr-MOF nanoparticles with a highly hydrophilic hyaluronic acid(HA)coating(PCN224-HA NPs)were prepared,and their carboxyl groups were activated to enable coupling with anti-CD45 antibodies through an amide reaction.High-purity CD8+T cells were then extracted from the spleens of mice,and the PCN224-HA NPs were loaded onto the T cells via the CD45 antigens on their surface.The coupling was observed using bioelectron microscopy and confocal microscopy,and free radical generation was detected under ultrasound irradiation.The viability of Cal27 cells was recorded by a microplate reader to explore therapeutic effects in vitro.Results·PCN224-HA NPs were successfully synthesized and confirmed to be coupled onto the surface of CD8+T cells,constructing a nanoengineered T cell system(T cells@PCN224-HA NPs).Free radical production was monitored after ultrasound irradiation and the viability of Cal27 cells decreased to 31.70%after in vitro treatment.Conclusions·Preliminary experiments demonstrated that the nanoparticles can be successfully loaded onto the surface of T cells,providing combined sonodynamic therapeutic effects from the nanoparticles and immunocidal effects from the T cells.This offers a novel strategy for the treatment of oral squamous cell carcinoma,with potential for further in vivo anti-tumor experiments and therapeutic applications.关键词
口腔鳞状细胞癌/声动力治疗/免疫治疗/纳米工程化T细胞体系/PCN224-HA纳米颗粒/CD8⁺ T细胞/协同治疗Key words
oral squamous cell carcinoma/sonodynamic therapy/immunotherapy/nanoengineered T cells/PCN224-HA nanoparticle/CD8+T cell/synergistic therapy分类
医药卫生引用本文复制引用
孟靖,谢玉婷,左佳鑫,熊屏..纳米工程化T细胞体系的构建及其对口腔鳞状细胞癌的体外治疗研究[J].上海交通大学学报（医学版）,2025,45(7):866-873,8.基金项目
上海市科学技术委员会基金(23ZR1438000) （23ZR1438000）
上海交通大学医学院附属第九人民医院学科群建设项目(JYJC202132). Project of Shanghai Science and Technology Commission(23ZR1438000) （JYJC202132）
Project for Disciplinary Group Construction of Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine(JYJC202132). （JYJC202132）
