上海海洋大学学报2025，Vol.34Issue(4)：749-759,11.DOI:10.12024/jsou.20240804613
饲料蛋氨酸水平对大口黑鲈稚鱼生长性能、消化能力和免疫抗氧化能力的影响
Effects of dietary methionine levels on growth performance,antioxidant capacity and intestinal development of juvenile largemouth bass(Micropterus salmoides)
摘要
Abstract
This experiment was conducted to study the effects of dietary methionine levels on the growth performance,digestion and absorption related indexes and antioxidant capacity of juvenile Micropterus salmoides.1 440 largemouth bass with an initial body weight of(0.18±0)g were selected and randomly divided into 6 groups(each group had 4 replicates,with 60 replicates each).They were raised in nylon cages with a water volume of 50 L in an outdoor aquaculture system,and fed with equal nitrogen and fat feed ranging from T1 to T6(methionine additions of 0,0.5％,1.0％,1.5％,2.0％and 2.5％,with measured values of 0.67％,1.08％,1.43％,1.78％,2.22％and 2.61％,respectively).The experimental period is 30 days.The results showed that as the feed methionine level increased,the weight gain rate,specific growth rate,and protein deposition of the experimental fish first increased and then decreased,and the feed coefficient first decreased and then increased.The weight gain rate and specific growth rate of the 1.08％,1.43％,1.78％,2.22％and 2.61％groups were significantly higher than the 0.67％group(P＜0.05),and the feed coefficient was significantly lower than the 0.67％group(P＜0.05);With the increase of feed methionine levels,the activity of trypsin in the intestinal tract of experimental fish showed a trend of first increasing and then decreasing,with the highest in the 1.78％group,and had no significant effect on other intestinal enzyme activities;With the increase of feed methionine levels,the activities of GSH-Px,CAT,T-SOD,and LZM in the liver of experimental fish first increased and then decreased.CAT,T-SOD,and LZM activities were highest at 1.78％,significantly higher than those in the 0.67％group(P＜0.05).Using specific growth rate as an evaluation indicator,the demand for methionine in juvenile largemouth bass is 1.72％,accounting for 3.23％of the feed protein.关键词
蛋氨酸/大口黑鲈/生长性能/抗氧化/肠道发育Key words
methionine/largemouth bass/growth performance/antioxidant/intestinal development分类
农业科技引用本文复制引用
李诗洋,陈晓瑛,梁浩辉,谢雨桐,胡俊茹,黄燕华,周萌,王国霞..饲料蛋氨酸水平对大口黑鲈稚鱼生长性能、消化能力和免疫抗氧化能力的影响[J].上海海洋大学学报,2025,34(4):749-759,11.基金项目
广州市基础研究计划民生科技专题(202002020018) （202002020018）
广东省重点领域研发计划(2021B0202050002) （2021B0202050002）
