世界科学技术-中医药现代化2025，Vol.27Issue(7)：1887-1897,11.DOI:10.11842/wst.20240518004
电针通过下调GCPII基因表达减轻大鼠创伤性脑损伤
Electroacupuncture Alleviates Traumatic Brain Injury in Rats by Down-Regulating GCPII Gene Expression
摘要
Abstract
Objective To study whether electroacupuncture(EA)attenuates traumatic brain injury(TBI)by down-regulating glutamate carboxypeptidase Ⅱ(GCPII)gene expression in rats.Methods Rats were divided into Sham group(n=10),TBI group(n=11),TBI+EA group(n=11),TBI+EA+GCPII-NC group(n=11)and TBI+EA+GCPII-OE group(n=11).Sham group rats underwent sham surgery,while other groups rats underwent TBI modeling using an electronic brain injury instrument.The rats in Sham group and TBI group were fed normally,and the rats in other groups were treated with electroacupuncture for 14 days.On the basis of electroacupuncture treatment,rats in TBI+EA+GCPII-NC group and TBI+EA+GCPII-OE group were injected with GCPII-NC and GCPII-OE,respectively.After treatment,the cognitive function of rats was evaluated by neurological function score and Morris water labyrinth task.HE and TUNEL staining were performed on brain tissue.The water content,glutamate(Glu)content,calcium(Ca2+)concentration,superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)in brain tissue of rats in each group were measured.The mRNA or protein expression of GCPII,Bax,Bcl2 and cleaved caspase-3 in brain tissue were detected by qRT-PCR or Western blot.Results Compared with Sham group,the expression of mRNA and protein of GCPII in TBI group increased,the neurological function score and escape latency increased,the number of crossing the platform decreased,the water content increased,the cortex showed obvious damage,the content of Glu increased,the concentration of calcium increased,the levels of SOD,CAT and GSH-Px decreased,the level of MDA increased,the positive rate of TUNEL increased,the expression of Bax and cleaved caspase-3 protein increased,and the expression of Bcl2 protein decreased significantly(P<0.05).Compared with TBI group,the expression level of mRNA and protein of GCPII in TBI+EA group decreased,the neurological function score and escape latency decreased,the number of crossing platform increased,the water content decreased,the morphology of cortex improved obviously,the content of Glu decreased,the concentration of calcium decreased,the level of SOD,CAT and GSH-Px increased,the level of MDA decreased,the positive rate of TUNEL decreased,the expression level of Bax and cleaved caspase-3 protein decreased,and the expression level of Bcl2 protein increased significantly(P<0.05).Compared with TBI+EA group and TBI+EA+GCPII-NC group,the expression level of mRNA and protein of GCPII in TBI+EA+GCPII-OE group increased,neurological function score and escape latency increased,the number of crossing platform decreased,water content increased,cortical injury aggravated,Glu content increased,calcium concentration increased,SOD,CAT and GSH-Px levels decreased,MDA level increased,TUNEL positive rate increased,Bax and cleaved caspase-3 protein expression increased,and the expression of Bcl2 protein decreased significantly(P<0.05).Conclusion By down-regulating the expression of GCPII gene,electroacupuncture reduced the content of glutamate in the brain tissue of TBI rats,thereby inhibiting calcium overload,oxidative stress and neuronal apoptosis in the brain tissue,and thereby improving cognitive function and alleviating brain injury.关键词
创伤性脑损伤/电针/谷氨酸羧肽酶Ⅱ/谷氨酸/钙超载/氧化应激/神经元凋亡Key words
Traumatic brain injury/Electroacupuncture/Glutamate carboxypeptidase Ⅱ/Glutamate/Calcium overload/Oxidative stress/Neuronal apoptosis分类
医药卫生引用本文复制引用
韦晓梅,段荣博,汤阳阳,吴涛..电针通过下调GCPII基因表达减轻大鼠创伤性脑损伤[J].世界科学技术-中医药现代化,2025,27(7):1887-1897,11.基金项目
国家自然科学基金委员会青年科学基金项目(81904310):电针基于AMPK/mTOR通路调节颅脑损伤后自噬与康复新机制,负责人:吴涛 （81904310）
陕西省科学技术厅陕西省自然科学基金委青年项目(2021JQ-729):基于细胞自噬研究电针调控颅脑损伤康复新机制,负责人:吴涛. （2021JQ-729）
