世界科学技术-中医药现代化2025，Vol.27Issue(7)：2063-2077,15.DOI:10.11842/wst.20240813004
马蓝中靛蓝和靛玉红生源合成途径上关键基因的筛选和验证
Mining and Identifying Key Genes on the Biosynthetic Pathway of Indigo and Indirubin in Baphicacanthus cusia(Nees)Bremek
摘要
Abstract
Objective To study mine and identify the key genes on the biosynthetic pathway of indigo and indirubin in Baphicacanthus cusia(Nees)Bremek.(B.cusia).Methods A weighted co-expression network analysis of transcriptome and metabolome data was conducted to screen out candidate genes.Selected genes were further cloned by homologous recombination in Escherichia coli and Saccharomyces cerevisiae to confirm their function.The LC-MS analysis was used to test the metabolic products.Results The enzyme encoded by gene FMO-EVM0009245 recombined in Escherichia coli could oxidize indole to indigo and indirubin,While the enzyme encoded by gene CYP-EVM0022856 and CYP-EVM0028891 recombined in Saccharomyces cerevisiae could oxidize indole to indigo.Conclusion This article lays a foundation for further elucidating the molecular mechanism of indigo and indirubin biosynthesis and provided research basis for increasing the content of medicinal components of B.cusia.关键词
马蓝/靛蓝/靛玉红/生源合成途径/重组克隆Key words
Baphicacanthus cusia(Nees)Bremek./Indigo/Indirubin/Biosynthetic pathway/DNA cloning by homologous recombination分类
医药卫生引用本文复制引用
韩笑雨,杨松,冯冲,闫建丽,赵志敏,杨得坡..马蓝中靛蓝和靛玉红生源合成途径上关键基因的筛选和验证[J].世界科学技术-中医药现代化,2025,27(7):2063-2077,15.基金项目
广东省云浮市科技局云浮市科技计划项目(2021020605):黑老虎等四种南药特色品种种植基地建设及生态种植推广,负责人:杨得坡 （2021020605）
云南省科技厅科技人才与平台计划(院士专家工作站)(202205AF150071):云南省杨得坡专家工作站,负责人:张丽霞. （院士专家工作站）
