世界林业研究2025，Vol.38Issue(2)：17-24,8.DOI:10.13348/j.cnki.sjlyyj.2025.0018.y
松树离体再生和遗传转化体系的研究进展
Research Progress in In Vitro Regeneration System and Genetic Transformation System for Pinus
摘要
Abstract
Pine is a commercially significant forest species.The conventional breeding methods currently in use are unable to meet the demand for improving pine germplasm.However,modem molecular breeding technology allows for the rapid cultivation of new pine germplasm with superior traits.The establishment of a pine genetic transformation system facilitates the understanding of pine gene functions and provides the basis for subsequent genetic improvement.The highly efficient regeneration system serves as the basis for the genetic transformation system.This paper reviews the research progress in establishing regeneration and genetic transformation systems for pine.It focuses on the two regeneration pathways of organogenesis and somatic embryogenesis and their influencing factors,Agrobacterium-mediated and gene gun-mediated genetic transformation and their influencing factors,and the molecular mechanisms regulating in vitro regeneration.The existing problems associated with pine's regeneration and genetic transformation systems are further examined.The review attempts to offer references for future research and investigation in this field.关键词
松树/器官发生/体细胞胚发生/农杆菌介导法/遗传转化Key words
pine/organogenesis/somatic embryogenesis/Agrobacterium mediated method/genetic transformation分类
农业科技引用本文复制引用
杨加松,秦雅琪,黄桂华,徐建民,赵霞,王溢,车晓亮,韩强..松树离体再生和遗传转化体系的研究进展[J].世界林业研究,2025,38(2):17-24,8.基金项目
科技创新2030—农业生物育种重大项目"西南地区松树优质高产新品种与培育"(2023ZD0405902). （2023ZD0405902）
