世界中医药2025，Vol.20Issue(10)：1672-1678,1685,8.DOI:10.3969/j.issn.1673-7202.2025.10.005
基于调控血管生成相关基因表达的理肺消积丸对肺癌小鼠肿瘤的抑制作用研究
Inhibitory Effect of Lifei Xiaoji Pills on Tumors in Lung Cancer Mice Based on the Regulation of Angiogenesis-related Gene Expression
摘要
Abstract
Objective:To investigate the inhibitory effect of Lifei Xiaoji Pills on tumor growth in a mouse model of lung cancer by regulating the expression of angiogenesis-related genes.Methods:A lung cancer model was established by subcutaneous inoculation of Lewis cells in 30 C57BL/6 mice,which were randomly divided into three groups according a random number table(n=10 per group):model group,Lifei Xiaoji Pills group,and cisplatin group.From the day following modeling,mice were treated according to group.The Lifei Xiaoji Pills group received drug decoction by gavage at 205 mg/(kg·d)and intraperitoneal injection of normal saline.The cisplatin group received intraperitoneal injections of 0.5 mg/kg cisplatin every other day and gavage with an equal vol-ume of double-distilled water.The model group received gavage with double-distilled water and intraperitoneal saline injection.After 2 weeks,the mice were euthanized and tumors were harvested.Tumor mass and volume were recorded.Histopathological changes in tumor tissues were observed using hematoxylin-eosin(HE)staining.Expression of vascular endothelial growth factor(VEGF)and microvessel density(MVD)were measured by immunohistochemistry.Differentially expressed angiogenesis-related genes were screened using a PCR array.Micro-RNA expression of angiopoietin-like protein 3(ANGPTL3),chemokine ligand 1(CXCL1),and collagen type Ⅳ alpha 3 chain(COL4A3)was assessed using real-time quantitative PCR(RT-qPCR).Protein expression of AN-GPTL3 and CXCL1 was analyzed by Western blot,and COL4A3 expression by immunofluorescence.Results:Compared with the model group,both the Lifei Xiaoji Pills and cisplatin groups exhibited significantly reduced tumor mass and volume(P＜0.01),im-proved tumor histopathology,significantly downregulated VEGF expression,and reduced MVD(P＜0.01).Thirteen differentially expressed angiogenesis-related genes were identified in the Lifei Xiaoji Pills group compared with the model group,including AN-GPTL3,CXCL1,and COL4A3,which are involved in epithelial cell proliferation and angiogenesis regulation.Expression of AN-GPTL3,CXCL1,and COL4A3 at both mRNA and protein levels was significantly downregulated in the Lifei Xiaoji Pills group com-pared with the model group(P＜0.05).Conclusion:Lifei Xiaoji Pills may inhibit tumor growth in lung cancer mice by downregu-lating angiogenesis-related genes such as VEGF,ANGPTL3,and CXCL1.关键词
理肺消积丸/非小细胞肺癌/血管生成/聚合酶链反应阵列/血管内皮生长因子/血管生成素样蛋白3/趋化因子配体1/Ⅳ型胶原α3链Key words
Lifei Xiaoji Pills/Non-small cell lung cancer/Angiogenesis/Polymerase chain reaction array/Vascular endothelial growth factor/Angiopoietin-like protein 3/Chemokine ligand 1/Collagen type Ⅳ alpha 3 chain分类
医药卫生引用本文复制引用
谷雨,刘素晓,何志远,丁锦泵,孔德琪,黄慧敏,冯素香,刘学芳,李亚..基于调控血管生成相关基因表达的理肺消积丸对肺癌小鼠肿瘤的抑制作用研究[J].世界中医药,2025,20(10):1672-1678,1685,8.基金项目
国家自然科学基金项目(82074403) （82074403）
国家中医临床研究基地科研专项(2022JDZX099) （2022JDZX099）
河南省医学科技攻关计划联合共建项目(LHGJ20230691) （LHGJ20230691）
河南省自然科学基金项目(232300420272) （232300420272）
河南省中医药科学研究专项(2022ZY1022) （2022ZY1022）
