世界中医药2025，Vol.20Issue(10)：1699-1704,1712,7.DOI:10.3969/j.issn.1673-7202.2025.10.009
糖肾宁抑制系膜增殖改善糖尿病肾病肾纤维化的作用机制
Mechanism of Tangshenning in Inhibiting the Proliferation of Mesangial Cells to Improve Renal Fibrosis in Diabetic Kidney Disease
摘要
Abstract
Objective:To investigate the mechanism by which Tangshenning(TSN)inhibits mesangial cell proliferation and amel-iorates renal fibrosis in diabetic kidney disease(DKD)based on the Yes-associated protein(YAP)/PDZ-binding motif transcrip-tional coactivator(TAZ)signaling pathway.Methods:A DKD mouse model was established by feeding KK-Ay mice a high-fat diet.Successfully modeled mice were randomly divided into three groups:model group,TSN group,and valsartan group,with C57BL/6J mice used as the normal control.After 10 weeks of continuous administration,body weight,blood glucose,blood urea nitrogen(BUN),serum creatinine(Scr),and urinary albumin-to-creatinine ratio(UAER)were measured.Renal histopathology was evalua-ted by hematoxylin-eosin(HE),periodic acid-Schiff(PAS),and Masson's trichrome(Masson)staining.Ultrastructural changes in glomeruli were observed by transmission electron microscopy.Protein expression levels of YAP,TAZ,TEA domain transcription fac-tor 1(TEAD1),connective tissue growth factor(CTGF),and type Ⅳ collagen(Col-Ⅳ)in renal tissues were detected using West-ern blot.Expression of fibronectin(FN)and laminin(LN)was assessed by immunohistochemistry.Results:Compared with the model group,TSN had no significant effect on body weight and blood glucose,but significantly reduced BUN and Scr levels.UAER also decreased significantly after treatment(P＜0.05).Protein expression levels of YAP,TAZ,TEAD1,CTGF,and Col-Ⅳ in renal tissue were significantly downregulated(P＜0.05).FN and LN expression levels were also reduced.Histological analysis revealed alleviation of mesangial matrix,mesangial cell proliferation,glycogen and collagen deposition,as well as reduction in glomerular basement membrane thickening,foot process fusion,and extracellular matrix accumulation in the mesangial area.Conclusion:TSN may inhibit mesangial cell proliferation and extracellular matrix accumulation by suppressing the YAP/TAZ signaling pathway,thereby improving renal fibrosis in DKD.关键词
糖尿病肾病/糖肾宁/细胞外基质增生/肾纤维化/系膜增殖/Yes相关蛋白/PDZ结合基序转录共激活因子/KK-Ay小鼠Key words
Diabetic kidney disease/Tangshenning/Extracellular matrix hyperplasia/Renal fibrosis/Mesangial proliferative/Yes-associated protein/PDZ-binding motif transcriptional coactivator/KK-Ay mouse分类
医药卫生引用本文复制引用
卢聪,单晓萌,王翠婷,朱智耀,邹大威,高彦彬..糖肾宁抑制系膜增殖改善糖尿病肾病肾纤维化的作用机制[J].世界中医药,2025,20(10):1699-1704,1712,7.基金项目
国家重点研发计划项目(2018YFC1704102)——东部地区名老中医学术观点、特色诊疗方法和重大疾病防治经验研究 （2018YFC1704102）
国家中医药管理局高彦彬全国名老中医专家传承工作室建设项目(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
