不同饲喂方式下大鳞副泥鳅的代谢组学特征分析OA

Paramisgurnus dabryanus,commonly known as Dabry's loach or large-scaled loach,has high nutritional value and serves as a main food source for endangered species such as crested ibises.In order to evaluate the safety of P.dabryanus as a feed source and investigate the effects of different feeding methods on its metabolism,metabolomic analysis was performed on Dabry's loaches using high performance liquid chromatography-mass spectrometry(HPLC-MS).The results showed that Dabry's loaches contained abundant nutrients,with a total of 680 identified compounds.Artificial feeding with commercial compound feed signi-ficantly altered the metabolic profile of Dabry's loaches,with 139 metabolites showing significant changes,mainly enriched in lipid metabolism pathways.Several steroid hormones including estrogens(estriol,17α-ethinylestradiol,and 18-hydroxycorticosterone)were found to accumulate significantly in the artificially fed Dabry's loaches,while the levels of male hormones,such as 17α-methyltestosterone,1,4-androstanedione,16α-hydroxytestosterone,and 11-ketotestosterone,were significantly reduced(P＜0.05).In addition,the levels of γ-tocopherol,linoleic acid,and α-linolenic acid were also significantly increased in artificially fed Dabry's loaches.This study provides a preliminary metabolic analysis of the nutritional value and food safety of P.dabryanus under different feeding methods,hoping to offer a theoretical basis for the development and uti-lization of Dabry's loaches as a feed resource for endangered species such as crested ibises.