水生生物学报2025，Vol.49Issue(8)：25-33,9.DOI:10.3724/1000-3207.2025.2025.0016
碳酸氢钠对慢性高温胁迫下大口黑鲈生长性能、血液生化和肠道损伤的影响
SODIUM BICARBONATE ON GROWTH PERFORMANCE,BLOOD BIOCHEMI-CAL PARAMERERS,AND INTESTINAL DAMAGE OF LARGEMOUTH BASS(MICROPTERUS SALMOIDES)UNDER CHRONIC HEAT STRESS
摘要
Abstract
In order to investigate the effect of sodium bicarbonate(NaHCO3)on intestinal damage in largemouth bass(Micropterus salmoides)under chronic high-temperature stress,juvenile largemouth bass(20.26±0.08)g were fed diets with or without sodium bicarbonate(NaHCO3,5 g/kg)for 8 weeks.The results showed that there was no significant difference in the specific growth rate in the NaHCO3 group compared to the control group at chronic high temperatures.However,the NaHCO3 group exhibited significantly improved feed efficiency,protein deposition rate,and protein effi-ciency,along with a marked increase in intestinal amylase and trypsin activities.The addition of NaHCO3 also signifi-cantly enhanced the intestinal total antioxidant capacity(T-AOC),catalase(CAT),superoxide dismutase(SOD),and glutathione peroxidase(GPx)activities.Furthermore,NaHCO3 up-regulated the expression of cat and gpx1a genes and down-regulated the gene expression of keap1.Meanwhile,the expressions of pro-inflammatory cytokines(il-1β,tnfα,and il15)were significantly down-regulated in the group,while the expression of anti-inflammatory cytokine il10 was up-regulated.In addition,the expression of hsp90,hsp70,and hsp60 genes in the intestines of largemouth bass in the NaHCO3 group was significantly lower than that in the control group.Histologic analysis of the intestinal tract showed that high-temperature stress induced intestinal damage in largemouth bass,and the addition of NaHCO3 significantly improved the intestinal tissue morphology and up-regulated the expression of intestinal zo1,jam,occludin,and muc2.In conclusion,the addition of 5 g/kg NaHCO3 to the feed effectively alleviated chronic high-temperature stress-induced intestinal damage in largemouth bass by improving intestinal antioxidant capacity,intestinal inflammation,and intesti-nal barrier function.These findings may provide a new perspective to improve the intestinal health of fish under high-temperature stress.关键词
碳酸氢钠/高温胁迫/生长性能/肠道健康/大口黑鲈Key words
NaHCO3/Heat stress/Growth performance/Gut health/Largemouth bass分类
农业科技引用本文复制引用
张瑞,解绶启,巫丽云,曹琨琨,金俊琰,杨云霞,张志敏,韩冬,刘昊昆,朱晓鸣..碳酸氢钠对慢性高温胁迫下大口黑鲈生长性能、血液生化和肠道损伤的影响[J].水生生物学报,2025,49(8):25-33,9.基金项目
国家重点研发计划(2022YFD2400800)资助[Supported by the National Key R&D Program of China(2022YFD2400800)] （2022YFD2400800）
