水生生物学报2025，Vol.49Issue(8)：44-52,9.DOI:10.3724/1000-3207.2025.2024.0485
南方碱蓬耐盐基因的挖掘及候选基因的功能验证
EXPLORATION OF SALT TOLERANCE GENES AND FUNCTIONAL VALIDA-TION OF CANDIDATE GENES IN SUAEDA AUSTRALIS
摘要
Abstract
Suaeda australis,a key halophytic species in southern China's coastal ecosystems,plays a vital role in stabi-lizing fragile ecosystems due to its remarkable salt tolerance.Addressing escalating coastal soil salinization,this study systematically investigated the molecular mechanisms underlying its salt adaptation through integrated physiological,transcriptomic,and functional validation approaches.Under different salt concentration treatments(ST1 and ST2),S.australis exhibited significantly enhanced activities of antioxidant enzymes(CAT,SOD,POD)alongside elevated malondialdehyde(MDA)and hydrogen peroxide(H2O2)levels,indicating activation of antioxidant defenses to miti-gate oxidative damage under high-salinity stress.Transcriptome profiling identified 2434 differentially expressed genes(1568 upregulated and 866 downregulated).Gene Ontology(GO)and KEGG enrichment analyses revealed these genes to be predominantly associated with antioxidant responses,osmotic regulation,signal transduction,and carbon metabolism.Additionally,a total of 146 transcription factors related to salt stress response were identified.The RTq-PCR validation results were consistent with transcriptome data,further confirming the critical role of these genes in the salt stress response.Notably,the MYB family gene Sau00119 was significantly upregulated in ST2,highlighting its crucial role in the salt tolerance mechanism of S.australis.These findings provide new insights into the salt tolerance mechanism of S.australis and offers potential gene targets for the genetic improvement of salt tolerant plants.关键词
盐胁迫/转录组/亚细胞定位/生理特性/南方碱蓬Key words
Salt stress/Transcriptome/Subcellular localization/Physiological characteristics/Suaeda australis分类
农业科技引用本文复制引用
李俊奇,樊佳鑫,钟颂焕,沈周洋,高天翔,瞿印权,张秀梅..南方碱蓬耐盐基因的挖掘及候选基因的功能验证[J].水生生物学报,2025,49(8):44-52,9.基金项目
国家重点研发计划重点专项(2023YFD2401903) （2023YFD2401903）
浙江省自然科学基金探索青年项目(LQ24D060004) （LQ24D060004）
浙江省大学生科技创新活动计划(新苗人才计划)(2024R411A006) （新苗人才计划）
浙江省省属高校科研院所基本科研业务专项资金(2024J001-1)资助[Supported by the National Key Research and Development Program of China(2023YFD2401903) （2024J001-1）
the Youth Project of Zhejiang Natural Sciences Foundation(LQ24D060004) （LQ24D060004）
the Program of"Xinmiao"(Potential)Talents in Zhejiang Province(2024R411A006) （Potential）
Basic Scientific Research Operating Funds for Provincial Universities and Research Institutes of Zhejiang Province(2024J001-1)] （2024J001-1）
