水生生物学报2025，Vol.49Issue(8)：97-106,10.DOI:10.3724/1000-3207.2025.2024.0440
模拟运输温度变化对中华鳖幼鳖应激响应及抗氧化能力的影响
SIMULATED TRANSPORT TEMPERATURE CHANGE ON STRESS RESPONSE AND ANTIOXIDANT CAPACITY OF JUVENILE CHINESE SOFT-SHELLED TURTLE PELODISCUS SINENSIS
摘要
Abstract
The Chinese soft-shelled turtle(Pelodiscus sinensis)is a special aquaculture species in China,known for its sensitivity to temperature changes.Stress response is easy to occur during long-distance transportation such as off-site feeding and cross-domain sales,which has adverse effects on the product quality and health.Suitable temperature can alleviate transportation stress.In order to investigate the effects of temperature change on stress response and antioxi-dant capacity during simulated transportation of juvenile P.sinensis,the experiment was carried out by changing the rotational speed and temperature of the constant temperature and humidity shaker to simulate the transportation process.In this study,Chinese soft-shelled turtles with initial weight of(200±10)g were simulated to be transported at high temperature(T1,32℃)and low temperature(T2,20℃)for 12h,and then left to recover at ambient temperature of 32℃for 12h after transportation.The results showed that during the transportation stage,compared with group T1,the rela-tive abundance of mRNA of liver heat shock protein(Hsp90,Hsp70),glutathione peroxidase 4(Gpx4),nuclear factor E2-related factor 2(Nrf2),and plasma cortisol(COR)in group T2 were significantly reduced(P<0.01).Compared with T1 and T2 in the transportation stage,the relative abundance of mRNA of Hsp70,manganese superoxide dismutase(Mnsod),glutathione peroxidase(Gpx1,Gpx4)in the liver and plasma COR content in the recovery stage were signifi-cantly reduced(P<0.05).In the recovery stage,compared with group T1,the relative abundance of mRNA of Hsp70,Hsp90,Gpx1,Gpx4,Nrf2,and SOD enzyme activity of group T2 were significantly reduced(P<0.05),while the proportion of type Ⅰ collagen and the ratio of type Ⅰ to type Ⅲ collagen in the calipash tissue were significantly increased(P<0.01).The results showed that simulated high-temperature transportation causes oxidative stress in Chinese soft-shelled turtles.Low-temperature transportation and recovery were beneficial to alleviate stress response,oxidative damage,and maintain the calipash form during transportation.This study provides scientific reference for establishing reasonable transportation regulations in the production of Chinese soft-shelled turtles.关键词
模拟运输/温度/静置恢复/应激/抗氧化/中华鳖幼鳖Key words
Simulated transport/Temperature/Recovery/Stress/Antioxidation/Juvenile Pelodiscus sinensis分类
农业科技引用本文复制引用
高劲涛,罗嘉翔,宋伟,钱国英,李彩燕..模拟运输温度变化对中华鳖幼鳖应激响应及抗氧化能力的影响[J].水生生物学报,2025,49(8):97-106,10.基金项目
浙江省农业重大技术协同推广计划(2022XTTGSC05) （2022XTTGSC05）
宁波市公益性研究计划(2024S133) （2024S133）
宁波市自然科学基金重点项目(2022J186) （2022J186）
宁波市科技创新2025重大专项(2021Z009)资助[Supported by the Zhejiang Province Agricultural Major Technology Collaborative Extension Program(2022XTTGSC05) （2021Z009）
Ningbo Public Welfare Research Program(2024S133) （2024S133）
Key Project of Ningbo Natural Science Foundation(2022J186) （2022J186）
the Major Technology Research and Development Project in Ningbo(2021Z009)] （2021Z009）
