水生态学杂志2025，Vol.46Issue(4)：1-11,11.DOI:10.15928/j.1674-3075.202501100002
藻菌共培养体系微生物生长特性及互作转录组分析
Microbial Growth Characteristics and Interaction Transcriptome Analysis of a Co-cultivation System of Microalgae and Fungi
摘要
Abstract
Microalgal-fungal co-cultivation has wide applications in many fields such as microalgae harvesting and wastewater treatment.In this study,a microalgal-fungal co-cultivation system was estab-lished using Chlorella sorokiniana and Aspergillus oryzae,and we analyzed the microbial growth char-acteristics and explored symbiotic relationships and regulatory mechanisms between the microalgae and fungi,aiming to optimize algal-fungal co-culture conditions.Five inoculation ratios of A.oryzae cells to C.sorokiniana spores(1:10,1:5,1:2,1:1,2:1)were prepared and the biomass,cellular content of biomolecules(lipids,proteins,and polysaccharides),and the microscopic morphology of A.oryzae and C.sorokiniana were observed after 96 h.The co-culture of C.sorokiniana and A.oryzae at the optimal inoculation ratio was then compared with axenic cultures of C.sorokiniana and A.oryzae.Differences in gene expression and the effects of microalgae-fungi interactions on growth and metabolism at the molecular level were clarified by interaction transcriptome analysis.The initial co-culture ratio of fungi to microalgae significantly influenced the biomass and biomolecular content of the co-cultivation system,and the optimal co-culture ratio was 1:1.After 96 h,fungi biomass exceeded microalgae biomass,and a decrease in the initial proportion of fungi resulted a reduction in the final total biomass.When the ratio of fungi to algae was reduced from 2:1 to 1:10,the overall biomass decreased from 0.47 g/L to 0.39 g/L.Microalgae were rich in lipids,proteins,and polysaccharides,and a decrease in the microalgae inocula-tion ratio reduced the cellular content of biomolecules.When the ratio of fungi spores to algae cells was increased from 1:10 to 2:1,intracellular polysaccharide content in the co-cultivation system signifi-cantly decreased,from 413.81 mg/g to 169.95 mg/g.SEM analysis revealed the C.sorokiniana ad-hered to A.oryzae hyphae,forming a three-dimensional structure that enhanced gas exchange and nutri-ent flow between C.sorokiniana and A.oryzae.Transcriptome analysis of mutual interactions indicated that,under co-cultivation conditions,the genes of C.sorokiniana related to methyltransferase and RNA synthesis were significantly upregulated,while the photosynthetic pigment genes were downregulated.This result indicates that co-culture enhances the proliferation of microalgae but reduces light utiliza-tion.In the co-cultivation system,carbohydrate metabolism and the TCA cycle of A.oryzae were inhibit-ed,indicating a competition between microalgae and fungi for organic matter in the co-cultivation system.This study elucidated microbial growth characteristics and interactions between microalgae and fungi in different co-cultivation systems,and provides technical guidance for the application of microalgal-fungal co-cultivation systems in wastewater treatment and microalgae biomass harvesting.关键词
索罗金小球藻/米曲霉/共培养/转录组学/生长特性Key words
Chlorella sorokiniana/Aspergillus oryzae/co-cultivation/transcriptomics/growth character-istics分类
资源环境引用本文复制引用
刘东阳,薛颖昊,朱联东..藻菌共培养体系微生物生长特性及互作转录组分析[J].水生态学杂志,2025,46(4):1-11,11.基金项目
湖北省自然科学基金(2022CFA070) （2022CFA070）
国家自然科学基金(22278323). （22278323）
