水生态学杂志2025，Vol.46Issue(4)：24-34,11.DOI:10.15928/j.1674-3075.202411240002
赤霉素和羧化多壁碳纳米管对不同藻处理体系去除养猪废水中抗生素性能的研究
Effects of Gibberellin(GAs)and Carboxylated Multi-Walled Carbon Nanotubes on the Removal Efficiency of Multiple Antibiotics in Wastewater by Bacteria-Microalgae-Fungi Symbiotic Systems
摘要
Abstract
Symbiotic systems formed by microalgae,bacteria,and fungi displays significantly enhanced antibiotic removal due to synergistic metabolism.In this study,we constructed a ternary symbiotic system comprised of Chlorella vulgaris,endophytic bacterium S395-2,and Clonostachys rosea.We then system-atically investigated the effects of adding Gibberellins(GAs)and carboxylated multi-walled carbon nano-tubes(MWCNTs)on the growth characteristics,photosynthetic activity,and removal efficiencies of six antibiotics[tetracycline hydrochloride(TC),aureomycin(CTC),ciprofloxacin(CPFX),norfloxacin(NFX),sulfadiazine(SMZ),sulfadimidine(SMX)]from this system.The aim of the study was to provide a novel strategy for the efficient treatment of wastewater from the swine industry.Four different bacteri-al-microalgae-fungal symbiotic systems were prepared for this study:Treatment 1,C.vulgaris+BG11 so-lution;Treatment 2,C.vulgaris+endophytic bacteria(S395-2);Treatment 3,C.vulgaris+C.rosea;Treat-ment 4,C.vulgaris+endophytic bacteria(S395-2)+C.rosea.Four concentrations of GAs(0,30,60,and 90 mg/L)were set for each treatment to compare antibiotic removal performance and determine the opti-mal GAs dosage.Then,using the optimal GAs concentration,four concentrations of MWCNTs:0,1.5,3.0,and 4.5 mg/L were set,and water samples were collected on day 3,7 and 10 to measure the antibiot-ic removal efficiency and determine the optimal MWCNTs dosage.Among the four treatment systems,the GAs concentration of 60 mg/L gave optimal growth-promoting effects across all four systems.In Treatment 4,the average daily productivity peaked at(0.248±0.017)g/(L·d),significantly higher than the control group at(0.193±0.018)g/(L·d)(P<0.01).However,when the GAs concentration was increased to 90 mg/L,growth rates declined in all systems,indicating an inhibitory effect.With the 60 mg/L GAs treatment,Chl-a content in Treatment 4 reached the peak value(91.54±7.16)µg/L on Day 7.The Fᵥ/Fm ratio(photosynthetic efficiency)in Treatment 4(0.81±0.08)was significantly higher than that of in Treatment 1(0.51±0.05),confirming that the light energy utilization efficiency was superior in the ternary symbiotic system of C.vulgaris+endophytic bacteria(S395-2)+C.rosea.Treatment 4 also had the best an-tibiotic removal performance for all six antibiotics(TC,CTC,CPFX,NFX,SMZ,and SMX),with respec-tive removal rates of 98.6%±1.12%,99.5%±0.36%,75.18%±4.37%,75.14%±2.16%,77.98%±5.48%and 80.24%±5.82%.Adding 1.5 mg/L MWCNTs to Treatment 4 with 60 mg/L Gas further enhanced the re-moval rates of the six antibiotics(TC,CTC,CPFX,NFX,SMZ,and SMX),with respective values of 99.25%±0.36%,99.59%±0.21%,79.26%±6.92%,78.34%±7.41%,82.55%±7.43%and 84.71%±7.75%.In conclusion,adding of 60 mg/L GAs and 1.5 mg/LMWCNTs in the ternary symbiotic system of C.vulgar-is+endophytic bacteria(S395-2)+C,rosea improved biomass production and the removal rate of antibiot-ics,and these results provide a useful reference for treating wastewater that contains antibiotics.关键词
共生/赤霉素/羧化多壁碳纳米管/抗生素去除/螺旋聚孢霉/内生细菌/养猪废水Key words
symbiosis/Gibberellins/carboxylated multi-walled carbon nanotubes/antibiotics removal/Clonostachysrosea/endophytic bacteria/wastewater from th swine industry分类
资源环境引用本文复制引用
秦媛媛,陆振杰,卢贝,严惠湘,韦静..赤霉素和羧化多壁碳纳米管对不同藻处理体系去除养猪废水中抗生素性能的研究[J].水生态学杂志,2025,46(4):24-34,11.基金项目
嘉兴市科技计划项目(2023AY11036,2022AY10015) （2023AY11036,2022AY10015）
嘉兴南湖学院科研项目(62204ZL) （62204ZL）
国家级大学生创新创业训练计划项目(202313291026). （202313291026）
