水生态学杂志2025，Vol.46Issue(4)：58-69,12.DOI:10.15928/j.1674-3075.202409130001
补藻模式对菌藻颗粒污泥形成及微生物群落结构的影响
Effect of Algae Replenishment on the Formation and Microbe Community Structure of Bacteria-Algae Granular Sludge
摘要
Abstract
In this study,we explored the effects of different algae replenishment strategies on sludge granule characteristics,pollutant removal efficiency,and microbial community composition,as well as the mechanisms of algal-bacterial granule formation in the sequencing batch reactor(SBR)system.The aim of the study was to reveal the mechanisms of algal-bacterial synergy under different augmentation strategies and provide a theoretical foundation for optimizing the algal-bacterial granular sludge process.Two algae replenishment strategies(treatment A:continuous algal feeding;treatment B:intermittent algal feeding)were set to culture bacteria-algae granular sludge,with synthetic wastewater as the input and chlorella as the algae species in the SBR system.When the algal-bacterial granules had matured,the mean granule sizes were(0.78±0.08)mm in treatment A and(0.97±0.05)mm in treatment B.The contents of chlorophyll-a were(4.83±0.04)mg/g in treatment A and(6.28±0.04)mg/g in treatment B,and the average removal rates of COD,NH4+-N and TP from the wastewater were,respectively,94.39%,87.99%,and 88.86%in treatment A and 94.45%,88.19%,and 91.12%in treatment B.Intermittent algae augmentation enhanced the pollutant removal capacity of algal-bacterial granular sludge,and facilitated the colonization of algae on the granule surface,increased algal cell growth,and stimulated the release of extracellular polymeric substances(EPS)by both algae and bacteria.The dominant bacteria genera in treatments A and B were Zoogloea,Thauera,Flavobacterium and Candidatus Accumulibacter(phosphorus accumulating bacteria).Thauera was the source of increased polysaccharide(PS)in the EPS.In addition,the formation of granular sludge in treatment A primarily depended on algal adhesion and growth,while treatment B utilized an algal barrier.The intermittent algae replenishment strategy for algae-bacteria granule cultiva-tion significantly shortened the formation of granules(10 days)and enhanced bacterial aggregation and degradation of carbonaceous pollutants.关键词
补藻模式/菌藻颗粒污泥/小球藻/微生物群落/形成机制Key words
algae-replenishment strategies/microbial algal granular sludge/chlorella/microbial com-munity/formation mechanism分类
资源环境引用本文复制引用
肖飞,贾壮壮,赵峰德,李林..补藻模式对菌藻颗粒污泥形成及微生物群落结构的影响[J].水生态学杂志,2025,46(4):58-69,12.基金项目
塔里木大学校长基金(TDZKSS202329 （）
TDZ-KSS202148) （）
国家自然科学基金项目(51969028) （51969028）
新疆生产建设兵团重大科技攻关项目(2018AA003). （2018AA003）
