中国生态农业学报（中英文）2025，Vol.33Issue(7)：1336-1347,12.DOI:10.12357/cjea.20240744
荫蔽环境对不同耐荫型大豆叶结构、生理特性及转录组的影响
Effects of shade environment on leaf structure,physiological characteristics,and transcriptome of different shade-tolerant varieties of soybeans
摘要
Abstract
To investigate the response of soybean varieties with varying shade tolerance to shade environment,this study used the strong shade-tolerant soybean variety'Changdou 33'(cd33)and the weak shade-tolerant soybean variety'Changdou 31'(cd31)as materials.Agronomic traits,leaf tissue structure,mesophyll ultrastructure,physiological and biochemical indicators,endogenous hor-mone content,and transcriptomics of different varieties of soybean under natural light and shade condition were analyzed.Under shading stress,both cd31 and cd33 exhibited increased plant height,reduced stem diameter,and decreased shoot dry matter,and the changes in cd33 were less pronounced than those in cd31.Soybean improved light utilization efficiency by reducing leaf thickness and palisade mesophyll thickness,while increasing mesophyll cell area,perimeter,and chloroplast area.Cd33 exhibited a more intact cellular structure,which mitigated the effects of shading on photosynthesis.Physiological and biochemical results indicated that un-der shade condition,superoxide dismutase and peroxidase activities increased;catalase activity decreased;soluble sugar and proline contents increased;soluble protein content decreased;malondialdehyde content declined;auxin,gibberellin,and abscisic acid con-tents decreased.This suggests that soybeans adapt to the environment by regulating their antioxidant systems,osmotic adjustment substances,and endogenous hormones.Additionally,the abscisic acid and gibberellin contents in cd33 leaves were lower than those in cd31 leaves,which may decrease plant height and contribute to stronger lodging resistance.Transcriptomic analysis further re-vealed that the strong shade-tolerant soybean variety had significantly more differentially expressed genes(5 783)under shade condi-tion than the weak shade-tolerant soybean variety(2 319).These differentially expressed genes were mainly enriched in pathways such as the hydrogen peroxide response,protein kinase activity,plant hormone signal transduction,and phenylpropanoid biosynthesis.In conclusion,the strong shade-tolerant soybean variety demonstrated greater shade tolerance by activating gene expression and meta-bolic pathways,and maintaining cellular stability and light energy utilization efficiency.关键词
大豆/遮荫/叶片结构/生理生化/内源激素/转录组Key words
soybean/shading/leaf structure/physiological and biochemical/endogenous hormones/transcriptome分类
农业科技引用本文复制引用
李丹,郭政,李万星,李小霞,曹晋军,靳鲲鹏,刘永忠..荫蔽环境对不同耐荫型大豆叶结构、生理特性及转录组的影响[J].中国生态农业学报（中英文）,2025,33(7):1336-1347,12.基金项目
山西农业大学生物育种工程(YZGC026)、国家科技创新2030-重大项目子课题(2023ZD040350503)、山西省育种联合攻关项目(YZGG-03-02)和山西省现代农业油料产业技术体系(2024CYJSTX05-09)资助 This study was supported by the Biological Breeding Engineering of Shanxi Agricultural University(YZGC026),the Sub-project of the National Sci-ence and Technology Innovation 2030 of China-Major Project(2023ZD040350503),Shanxi Provincial Joint Breeding Project(YZGG-03-02),and the Modern Agricultural Oil Crop Industry Technology System of Shanxi Province(2024CYJSTX05-09). （YZGC026）
