生物安全学报（中英文）2025，Vol.34Issue(3)：282-294,13.DOI:10.3969/j.issn.2095-1787.20240055
基于斜纹夜蛾胸部转录组的飞行肌蛋白及能量代谢酶基因分析
Analysis of flight muscle protein and energy metabolism enzyme genes based on the thoracic transcriptome of Spodoptera litura
摘要
Abstract
[Aim]Spodoptera litura is a long-distance migratory pest.The aim of this study was to elucidate its flight protein gene ex-pression in relation with its energy metabolic system.[Method]Based on adult thoracic transcriptome and gene function annotations,the structural proteins of the flight muscles,energy metabolism pathways and key enzymes in S.litura were elucidated.[Result]There are 10 different families of flight muscle structural proteins were annotated in S.litura.Among them,the numbers of transcripts for my-osin and actin are relatively large,with 29 and 43 transcripts,respectively.Additionally,a flightin coding gene was discovered.The flightin protein exhibited six specific motifs,with motif 1 displaying distinctive amino acid characteristics at specific loci in Lepidoptera insects.A total of 33 key enzyme genes involved in flight energy metabolism were annotated in S.litura.Based on these genes,three possible pathways for flight energy metabolism were constructed:the glycolysis pathway(comprising 15 enzymes),the fatty acid path-way(comprising 7 enzymes),and the proline oxidation pathway(comprising 4 enzymes).The products of these three pathways ulti-mately enter the tricarboxylic acid cycle(TCA cycle)through 7 enzymes to generate energy.Furthermore,two isomers of 3-phospho-glycerol dehydrogenase(GPDH)were annotated in S.litura.The flight muscles GPDH isoforms of Drosophila contain a specific C-ter-minal tripeptide,while the GPDH isoforms of Lepidoptera insects such as S.litura have a conserved C-terminal tetrapeptide,indicating species-specific evolution of GPDH.[Conclusion]This study provides basic data for the analysis of structural proteins and energy me-tabolism mechanisms of S.litura,and molecular information for the control of migratory pests.关键词
斜纹夜蛾/飞行肌蛋白/能量代谢酶/飞行能量代谢途径Key words
Spodoptera litura/flight muscle structural proteins/energy metabolism enzymes/flight energy metabolism pathways引用本文复制引用
张焱,付艳,孙和伟,徐进,付文..基于斜纹夜蛾胸部转录组的飞行肌蛋白及能量代谢酶基因分析[J].生物安全学报（中英文）,2025,34(3):282-294,13.基金项目
云南省基础研究重点项目(202201AS070025) （202201AS070025）
云南省重点领域科技计划项目(202001BB050002) （202001BB050002）
云南盘龙特色林果科技小院项目 （）
