生物技术通报2025，Vol.41Issue(7)：95-105,11.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0109
基于WGCNA鉴定大豆抗大豆花叶病毒NAC转录因子及其诱导表达分析
Identification and Induced Expression Analysis of Transcription Factors NAC in Soybean Resistance to Soybean Mosaic Virus Based on WGCNA
摘要
Abstract
[Objective]To identify the hub genes of transcription factors associated with soybean resistance to soybean mosaic virus(SMV),which may provide a theoretical foundation for elucidating molecular mechanism and developing resistant germplasm.[Method]Using the transcriptome data of soybean disease-resistant material X149 and disease-susceptible material X97 induced by SMV SC15,PlantTFDB v5.0 database was used to predict the transcription factor genes of the whole genome.Weighted gene co-expression network analysis(WGCNA)was applied to identify the resistance-associated modules and hub transcription factors based on differentially expressed transcription factor genes.String 12.0 was for predicitng transcription factor interacting proteins.RT-qPCR was to analyze the expression patterns of transcription factor genes induced by hormones(ETH,SA,MeJA and ABA).[Result]The whole genome contained 3 170 genes encoding transcription factors,of which 1 727 were differentially expressed.WGCNA analysis revealed that 1 727 genes were divided into six co-expression modules.Notably,the brown and turquoise modules demonstrated significant correlations with X149 resistance.Within the brown and turquoise modules,GmNAC030 in turquoise module,a NAC transcription factor,showed the highest connectivity and was identified as a core transcription factor.Other NAC transcription factor genes in the turquoise module expression were similar to GmNAC030 included GmNAC043,GmNAC085,GmNAC092,and GmNAC101.Prediction of interacting protein indicated that the interacting proteins of GmNAC030,GmNAC043,and GmNAC092 were primarily transcription factors.Specifically,only the interacting proteins Glyma.02g131700(bZIP1),Glyma.16g164800(AP2-EREBP),and Glyma.08G118200(WRKY48)were located within the module.RT-qPCR analysis demonstrated that GmNAC030 expression was predominantly induced by MeJA,while the other four NAC transcription factors were mainly induced by both MeJA and ETH.[Conclusion]The expression of five NAC transcription factors,GmNAC030,GmNAC043,GmNAC085,GmNAC092 and GmNAC101,is associated with soybean resistance to SMV and can be induced by MeJA and ETH.关键词
大豆/大豆花叶病毒/加权共表达网络分析/NAC转录因子/表达分析Key words
soybean/soybean mosaic virus/WGCNA/NAC transcription factor/expression analysis引用本文复制引用
牛景萍,赵婧,郭茜,王书宏,赵晋忠,杜维俊,殷丛丛,岳爱琴..基于WGCNA鉴定大豆抗大豆花叶病毒NAC转录因子及其诱导表达分析[J].生物技术通报,2025,41(7):95-105,11.基金项目
山西省农业关键核心技术攻关项目(NYGG27-4),山西省科技重大专项计划"揭榜挂帅"项目子课题(202201140601025-3-06),科技创新2030-重大项目(2023ZD04035),山西省现代农业产业技术体系建设项目(2024CYJSTX05-07),山西农业大学科技创新基金项目(2018YJ29) （NYGG27-4）
