生物技术通报2025，Vol.41Issue(7)：106-116,11.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1077
大麦HvERECTA基因的克隆及功能分析
Cloning and Preliminary Functional Analysis of HvERECTA Gene in Hordeum vulgare
摘要
Abstract
[Objective]Transmembrane protein ERECTA,a protein kinase(LRR-RLK)rich in leucine receptor-like serine/threonine,controls cell proliferation,regulates stomatal structure and responds to various stresses.The aim of this study is to elucidate the structure and functional expression of the HvERECTA gene in barley and lay a foundation for its biological function identification and crop molecular breeding.[Method]Using barley Morex as material,the HvERECTA gene of barley was cloned and its biological information and gene function were analyzed.RT-qPCR was used to analyze the functional characteristics of barley HvERECTA gene in different organs and under different stresses,and to study the expression patterns of HvERECTA gene in barley and typical bHLH-transcription factors HvSPCH,HvMUTE and HvFAMA related to barley stomatal development.[Result]The length of open reading frame of barley HvERECTA was 2 934 bp,encoding 977 amino acids,having typical LRR-RLK structure,localization predicted in the plasma membrane and containing signal peptide.The phylogenetic tree showed that it belonged to the ERECTA subfamily of Gramineae,and protein interaction predicted that it was a key gene regulating stomatal development.The results of RT-qPCR showed that the expression of HvERCTA was the highest in early leaves and lowest in the roots,and significantly different in abiotic stress of ABA,high temperature,low temperature and simulated drought(20%PEG6000).Under simulated drought stress,the gene expression of HvERECTA and HvSPCH showed similar trend,but opposite trend with the expression of HvMUTE gene.[Conclusion]HvERECTA gene is cloned from barley.It is a key gene to regulate the stomatal development.It is expressed in early leaves of barley,participates in ABA signal transduction pathway,and responds the abiotic stresses such as high-or low-temperature and drought.关键词
大麦/气孔发育/ERECTA基因/非生物胁迫/基因表达分析Key words
Hordeum vulgare/stomatal development/ERECTA gene/abiotic stress/gene expression analysis引用本文复制引用
韩燚,侯昌林,唐露,孙璐,谢晓东,梁晨,陈小强..大麦HvERECTA基因的克隆及功能分析[J].生物技术通报,2025,41(7):106-116,11.基金项目
河北省高等学校科学技术研究项目(YJZ2024001),天津市大学生创新创业训练计划(202410061101),天津市科技计划(22ZXZYSN00020,22ZYCGSN00020),东西部科技协作专项(津甘合作、鲁甘合作)(24CXNL004),天津市自然科学基金项目(22JCQNJC01470) （YJZ2024001）
