山西农业大学学报（自然科学版）2025，Vol.45Issue(4)：3-15,13.DOI:10.13842/j.cnki.issn1671-8151.202503008
谷子HMA基因家族的全基因组表征及其功能预测
Genome-wide characterization and functional prediction of the heavy metal ATPase(HMA)gene family in foxtail millet
摘要
Abstract
[Objective]Heavy metal ATPase(HMA)proteins play critical roles in the absorption and transport of various metal ions in plants.This study aimed to characterize the HMA gene family in foxtail millet through genome characterization,expres-sion profiling,and homology analysis to elucidate its functions.[Method]HMA gene family members in foxtail millet were identified using protein homology alignment and conserved domain screening.Bioinformatics analyses were performed to exam-ine their physicochemical properties,gene family expansion,conserved motifs,phylogenetic relationships,and protein molecu-lar docking.Transcriptomic and qRT-PCR analyses under Cd and Fe stress treatments were conducted to assess metal-respon-sive expression patterns.Functional predictions for selected SiHMA were inferred through homology with rice.[Results]Twelve HMA genes were identified and classified into two subgroups.Family expansion involved six HMA genes through two tandem duplication events(four genes,33.3%)and one segmental duplication event(two genes,16.7%).SiHMA showed tis-sue-specific responses to Cd and Fe stress.The possible functions of SiHMA were predicted.Functional predictions suggested that SiHMA5 mediated Zn,Cu,Pb and Cd efflux,while SiHMA10 might be involved in the loading and transportation of Cu in plants.[Conclusion]Tandem and segmental duplication drove HMA family expansion and functional diversification in foxtail millet.Cis-acting elements related to plant hormones and stress responses were identified in SiHMA promoters.Based on ho-mology,expression patterns,and previous evidence,we proposed roles for SiHMA1,SiHMA5,SiHMA6 and SiHMA10 in metal homeostasis.These results provide a theoretical basis for further study on HMA genes in foxtail millet.关键词
谷子/HMA基因家族/同源比较/表达特征/功能预测Key words
Foxtail millet/HMA gene family/Homologous analysis/Expression profiling/Functional prediction分类
农业科技引用本文复制引用
王心悦,陈畅,李康平,黄绒,王君,侯思宇,杨阳,韩渊怀..谷子HMA基因家族的全基因组表征及其功能预测[J].山西农业大学学报（自然科学版）,2025,45(4):3-15,13.基金项目
中央引导地方科技发展资金(YDZJSX2022B007) （YDZJSX2022B007）
国家自然科学基金项目(U21A20216) （U21A20216）
山西省研究生科研创新项目(2023KY327) （2023KY327）
