山西农业大学学报（自然科学版）2025，Vol.45Issue(4)：37-45,9.DOI:10.13842/j.cnki.issn1671-8151.202503032
基于BSA-seq的高粱柄伸长度QTL定位
QTL mapping of sorghum peduncle exsertion length using BSA-seq
摘要
Abstract
[Objective]This study aimed to identify quantitative trait loci(QTL)associated with sorghum peduncle length using BSA-seq technology,screen sorghum plants with excellent peduncle elongation traits,and provide a theoretical basis for im-proving elite sorghum backbone lines and breeding mechanization-adapted varieties.[Methods]The study used sorghum main-tainer lines 7050B(short peduncle)and Tx2925B(long peduncle),along with their F2 population derived from emasculated hy-bridization.Peduncle length was measured in parents and the segregating population.Two bulked pools,EL-L(long pedun-cle)and EL-S(short peduncle),were constructed for QTL mapping.[Results]The peduncle length of 7050B was significantly shorter than that of Tx2925B,with a phenotypic variation coefficient variation of 76.1%in the F2 population.SNP variations detected in EL-L,EL-S,7050B,and Tx2925B were 1 574 836,2 041 538,2 512 953 and 688 446 respectively.At a 99%confidence level,16 QTLs were identified on chromosomes 1,4,5,8 and 10,including 9 on chromosome 1,4 on chromo-some 4,and 1 each on chromosomes 5,8 and 10.The candidate intervals contained 2 to 1,401 genes,with 2748 and 1779 transcripts annotated in GO and KEGG databases,respectively.[Conclusion]This study identified 16 QTLs significantly asso-ciated with peduncle length across five sorghum chromosomes,providing candidate gene resources for deciphering the genetic mechanisms of peduncle elongation.These findings establish a molecular foundation for breeding mechanized sorghum varieties and improving elite germplasms.关键词
高粱/柄伸长度/遗传分析/QTLKey words
Sorghum/Peduncle Elongation Length/Genetic Analysis/QTL分类
农业科技引用本文复制引用
王佳旭,赵泽阳,杨琳琳,陈柏志,柯福来,张飞,朱凯,张旷野..基于BSA-seq的高粱柄伸长度QTL定位[J].山西农业大学学报（自然科学版）,2025,45(4):37-45,9.基金项目
抗逆高粱种质挖掘与核心种质构建(2023020405-JH/102-01-03) （2023020405-JH/102-01-03）
辽宁省农业科学院院长基金项目(2022BS0705) （2022BS0705）
沈阳市种业创新专项(24-215-2-02) （24-215-2-02）
国家现代农业产业技术体系高粱产业辽宁创新团队 （）
高粱生物育种(2025XTCX0401-3-3) （2025XTCX0401-3-3）
国家谷子高粱产业技术体系(CARS-06-14.5-A22) （CARS-06-14.5-A22）
