实验科学与技术2025，Vol.23Issue(4)：25-32,8.DOI:10.12179/1672-4550.20240050
酵母醇脱氢酶提纯教学实验的优化
Optimization of Teaching Experiment of Yeast Alcohol Dehydrogenase Extraction and Purification
摘要
Abstract
There are some problems in the teaching experiment of yeast alcohol dehydrogenase(YADH)extraction and purification.In order to obtain YADH with high purity,high activity,and high recovery,a single factor experiment is applied to optimize the separation and purification process of enzyme protein,including hot extraction,thermal denaturation precipitation,and organic solvent precipitation,which will improve the extraction and purification efficiency of YADH.The results show that the best effect of separation and purification is achieved when the solid-liquid ratio is 1∶6,the crude extraction temperature is 25℃,the crude extraction time is 1.5 h,the thermal denaturation temperature is 65℃,the thermal denaturation time is 15 min,the organic solvent precipitant is ethanol,the adding proportion of precipitated impure protein is 0.5 times of the supernatant,and the adding proportion of precipitated enzyme protein is 0.45 times of the supernatant.Through the optimization of the above conditions,the effect of teaching experiment is improved.It is also helpful to exercise students'practical skills,innovative thinking,and scientific research abilities.关键词
酵母醇脱氢酶/提取/纯化/实验教学/优化设计Key words
yeast alcohol dehydrogenase(YADH)/extraction/purification/teaching experiment/optimization design分类
生物科学引用本文复制引用
顾华杰,缪天,刘恒蔚,陈宏伟,郭伟强,王桃云..酵母醇脱氢酶提纯教学实验的优化[J].实验科学与技术,2025,23(4):25-32,8.基金项目
2024年苏州科技大学校级品牌课程建设项目(2024KCXX-06) （2024KCXX-06）
苏州科技大学课程思政示范项目(2021). （2021）
