实验科学与技术2025，Vol.23Issue(4)：33-37,53,6.DOI:10.12179/1672-4550.20240121
琥珀酸脱氢酶的竞争性抑制作用综合实验设计
Design of a Comprehensive Experiment on Competitive Inhibition in Succinate Dehydrogenase
摘要
Abstract
To enhance students'biochemical experimental operation and analysis skills,a comprehensive design and exploration is conducted on the competitive inhibition of succinate dehydrogenase.This involves improving the enzyme extraction method,optimizing the reaction system,determining the enzymatic reaction conditions,and adding experimental controls.The result show that using muscle homogenate obtained by grinding muscle and removing red blood cells as the improved crude enzyme extract eliminated color interference make the phenomenon of competitive inhibition more apparent.The ratio of crude enzyme extract,succinic acid and methylene blue is fixed in the enzymatic reaction system.The reaction is carried out at pH 7.4 and 37℃for 15 min,with the addition of a succinate-free control.This results in good experimental repeatability.The comprehensive design of the experiment is beneficial for students to understand the principles and characteristics of competitive inhibition of enzymes,as well as the factors that affect enzyme activity.关键词
琥珀酸脱氢酶/竞争性抑制作用/综合实验设计/生物化学实验教学Key words
succinate dehydrogenase/competitive inhibition/comprehensive experiment design/biochemistry experimental teaching分类
生物科学引用本文复制引用
宋佳蕾,曾玲芝,徐昌君,杨长福,杨奕樱..琥珀酸脱氢酶的竞争性抑制作用综合实验设计[J].实验科学与技术,2025,23(4):33-37,53,6.基金项目
贵州省科技计划项目(Qian kehe ZK[2022]485) （Qian kehe ZK[2022]485）
贵州省高等学校本科教学内容和课程体系改革项目(GZJG2024168) （GZJG2024168）
贵州中医药大学本科教学质量与教学改革项目(JG2022016). （JG2022016）
