实用医学杂志2025，Vol.41Issue(13)：1997-2003,7.DOI:10.3969/j.issn.1006-5725.2025.13.008
1型糖尿病大鼠皮质神经元与突触数量变化对认知功能的影响
Effects of cortical neuronal and synaptic number changes on cognitive function in rats with type 1 diabetes mellitus-unbiased stereoscopic study
摘要
Abstract
Objective To explore the alterations in the numbers of cortical neurons and synapses in type 1 diabetic rats and their correlation with cognitive dysfunction.Methods A type 1 diabetes model was induced by intraperitoneal injection of streptozotocin(STZ).Four-month-old specific pathogen-free(SPF)-grade Sprague-Dawley(SD)rats were randomly allocated into a control group and a diabetic group,with five rats in each group.After three months of continuous feeding,optical fractionator stereology was employed to quantitatively analyze the volume of the cerebral cortex,the number of neurons,and the number of synapses.Results In comparison with the control group,the volume of the cerebral cortex in the diabetic group decreased by 6.00%,and the number of neurons decreased by 14.09%.However,these differences were not statistically significant(P>0.05).Never the-less,the density of synapses significantly decreased by 70.14%(P<0.05),and there was a significant difference in the number of Spinophilin/Neurabin-positive synaptic boutons per neuron in the cortex(P<0.05).Conclusion Although early-stage type 1 diabetes does not lead to a significant loss of cortical neurons,the substantial reduction in synaptic numbers might be a crucial pathological basis for cognitive dysfunction.关键词
1型糖尿病/认知功能障碍/大脑皮质/体视学定量/神经元/突触Key words
type 1 diabetes/cognitive dysfunction/cerebral cortex/stereology/neurons/syn-apses分类
医药卫生引用本文复制引用
赵圆宇,谭敏,赵慧,杨静,赵丰,杜江..1型糖尿病大鼠皮质神经元与突触数量变化对认知功能的影响[J].实用医学杂志,2025,41(13):1997-2003,7.基金项目
国家自然科学基金面上项目(编号:82474299) （编号:82474299）
教育部人文社科项目(编号:23YJA840013) （编号:23YJA840013）
四川省老年学学会项目(编号:24SCLN004) （编号:24SCLN004）
