时珍国医国药2025，Vol.36Issue(13)：2446-2452,7.DOI:10.70976/j.1008-0805.SZGYGY-2025-1307
基于PI3K/AKT信号通路探讨当归芍药散抑制高糖诱导的HK-2细胞EMT的作用机制
Investigating Danggui Shaoyao San(当归芍药散)in inhibiting high glucose-induced EMT in HK-2 cells by based on the PI3K/AKT signaling pathway
摘要
Abstract
Objective To explore the mechanism by which Danggui Shaoyao San(当归芍药散,DSS)serum inhibits high glucose-in-duced epithelial-mesenchymal transition(EMT)in HK-2 cells through the PI3K/AKT signaling pathway.Methods DSS serum was prepared and used to induce high glucose in human renal tubular epithelial HK-2 cells.The optimal serum concentration was screened using the CCK-8 assay.There were two experiments.Experiment 1 included the normal group,high glucose group,DSS serum group,and blank serum control group;Experiment 2 was added with an AKT agonist group and a PI3K inhibitor group.In Experiment 1,Tran-swell assays were performed to detect cell migration ability,RT-qPCR was used to measure mRNA expression of Vimentin and E-cad-herin,and Western blot(WB)was used to measure protein levels of relevant markers.Results 20％volume fraction of DSS significantly affected HK-2 cell viability and inhibited migration.Compared with the high glucose group,the DSS serum group showed an increase in E-cadherin expression,and a decrease in Vimentin expression as well as the p-PI3K/PI3K and p-AKT/AKT ratios(P＜0.05).In Experiment 2,after the addition of AKT agonist and PI3 K inhibitor,it was further confirmed that DSS could inhibit EMT in renal tubular epithelial cells through the PI3K/AKT signaling pathway.Conclusion DSS effectively inhibits EMT of renal tubular epithelial cells by suppressing the PI3K/AKT signaling pathway.关键词
当归芍药散/糖尿病肾病/PI3K/AKT信号通路/上皮-间充质转化Key words
Danggui Shaoyao San(当归芍药散)/Diabetic nephropathy/PI3K/AKT signaling pathway/Epithelial-mesenchy-mal transition分类
医药卫生引用本文复制引用
郝傲寒,杨晓林,宋凡,李矜姚,宋恩峰,项琼,梅莎莎,王子明,夏振忠,杨静一,刘汗青,马丹,王博文..基于PI3K/AKT信号通路探讨当归芍药散抑制高糖诱导的HK-2细胞EMT的作用机制[J].时珍国医国药,2025,36(13):2446-2452,7.基金项目
湖北省卫健委中医药科研项目(ZY2019Z005) （ZY2019Z005）
国家中医药管理局全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
