时珍国医国药2025，Vol.36Issue(13)：2453-2460,8.DOI:10.70976/j.1008-0805.SZGYGY-2025-1308
基于肠道菌群和代谢组学探究二至丸延缓衰老的作用机制
Exploring the mechanism of Er'zhi Pill(二至丸)in delaying aging based on gut microbiota and metabolomics
摘要
Abstract
Objective To analyze the mechanism of Er'zhi Pill(二至丸,EZP)in delaying aging by combining gut microbiota and metabolomics.Methods A natural aging rat model was established and intervened with EZP-containing feed.Enzyme-linked immu-nosorbent assay(ELISA)was used to detect changes in superoxide dismutase(SOD)and malondialdehyde(MDA).Hepatic and renal tissues were observed using hematoxylin-eosin(HE)staining.16S rDNA sequencing technology was utilized to explore the changes in gut microbiota.Ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS)was employed for metabolomics analysis.Spearman correlation analysis was conducted to investigate the correlation between metabolites and gut microbiota.Results Compared with the natural aging model group,the MDA content in the medium-dose and low-dose groups of EZP was significantly reduced(P＜0.05),and no significant pathological changes were observed in the liver and kidney tissues.The 16S rDNA results showed that the relative abundance of the beneficial bacteria such as Blautia,Faecalibaculum,and Lactobacillus was increased by EZP.Identified by UPLC-Q-TOF-MS,the 13 differential metabolites related to aging were mainly enriched in 19 path-ways such as arachidonic acid(AA)metabolism and glycerophospholipid(GP)metabolism.Spearman analysis showed that some metab-olites were significantly correlated with specific gut microbiota.Conclusion EZP may delay aging by improving gut microbiota composi-tion and influencing AA and GP metabolisms.关键词
衰老/二至丸/代谢组学/肠道菌群Key words
Aging/Er'zhi Pill(二至丸)/Metabolomics/Gut microbiota分类
医药卫生引用本文复制引用
洪伟,张婉晴,吕心雨,刘红宁,单思..基于肠道菌群和代谢组学探究二至丸延缓衰老的作用机制[J].时珍国医国药,2025,36(13):2453-2460,8.基金项目
江西省卫生健康委科技计划(202211418) （202211418）
江西省自然科学基金面上项目(20232BAB206147) （20232BAB206147）
江西中医药大学博士科研启动基金课题(2019WBZR010) （2019WBZR010）
江西中医药大学校级科技创新团队发展计划(CXTD22007) （CXTD22007）
