生态环境学报2025，Vol.34Issue(8)：1265-1272,8.DOI:10.16258/j.cnki.1674-5906.2025.08.011
不同铝处理下纳米硅矿化沉积对豌豆根边缘细胞耐铝性的影响
Extracellular Silica Nanocoat Confers Aluminum Resistance in Root Tip and Root Border Cells of Pea under Different Aluminum Treatments
摘要
Abstract
Root border cells(RBCs)of pea were selected as model cells to study the physiological mechanisms and factors influencing silicon alleviation of aluminum(Al)toxicity.Polyvinylimine was used to induce the active biomineralization of aluminum and form nano silicon shells.The physiological mechanisms and effects of different aluminum solutions,such as aluminum chloride,aluminum nitrate,and aluminum sulfate,on the deposition of nano silicon on RBCs walls were studied.The results were as follows:1)nanosilica can increase the cell viability of RBCs,reduce the entry of reactive aluminum into the cells,lower the level of intracellular reactive oxygen species,and increase the mitochondrial membrane potential(the potentials of aluminum chloride,aluminum nitrate,and aluminum sulfate films increased by 25.68%,16.80%,and 17.33%,respectively)to reduce the toxic effect of aluminum on RBCs under different environmental conditions.2)Acid ions in different Al solutions can affect the absorption peak intensity of functional groups in cell wall components,with characteristic peaks at 3380,2940,1730,1650,1540,1460,1170,and 1050 cm-1.However,the absorption peak intensity decreased after nano-Si treatment,indicating that under Al toxicity stress,nano-Si treatment reduced the absorption peak intensity of carboxyl,hydroxyl,and carbonyl groups.3)Under different aluminum conditions,a nanosilicon layer can be formed on the cell wall of RBCs under PEI induction.Therefore,the mineralized deposition of nano silicon on cells can alleviate the toxic effects of Al ions.The type and concentration of ions can affect the changes in functional groups in the cell wall components of RBCs and the structure of silicon aluminum deposition.关键词
豌豆/铝毒/纳米硅/生物硅化/根边缘细胞Key words
pea/aluminum toxicity/nanosilica/biosilicification/root border cells分类
资源环境引用本文复制引用
冯英明,唐娇,陈醒韵,郭依唯,郑雨欣,田晓,喻敏..不同铝处理下纳米硅矿化沉积对豌豆根边缘细胞耐铝性的影响[J].生态环境学报,2025,34(8):1265-1272,8.基金项目
国家自然科学基金项目(42077150 （）
42377042) （）
