太原理工大学学报2025，Vol.56Issue(4)：656-664,9.DOI:10.16355/j.tyut.1007-9432.20240330
低温氧化煤生物降解产甲烷机理研究
Mechanism of Biodegradation and Methane Production from Low-temperature Oxidized Coal
摘要
Abstract
[Purposes]The goaf,as the primary residual space following coal mining,boasts a vast and expansive internal space and a rich residual coal resources.This makes it an important poten-tial site for implementing coal-bed methane bioengineering.Of particular note is that residual coal can theoretically significantly enhance the production of biomethane after low-temperature oxidation treat-ment.However,there is a significant gap in the current research on the biodegradation of coal residue in the goaf.[Methods]By carrying out the experiments of coal biodegradation and methane production at three different low-temperature oxidation levels,namely,low(LOC),medium(MOC)and high(HOC),the influence of coal oxidation level on methane production was analyzed,and the mechanism of low-temperature oxidized coal biodegradation and methane production was explored from the struc-ture of coal,the structure of microbial community,and metabolism products.[Results]The results demonstrate a positive correlation between biomethane production and the degree of oxidation within the temperature range of 80～180℃.Furthermore,methane production of oxidized coal is observed to be 29.63%～202.96%higher than that of original coal.After low-temperature oxidation,the struc-ture of coal undergoes a significant transformation,with the proportion of C-O functional groups ex-hibiting an increase of 7.60%～206.78%relative to original coal.Additionally,the degree of branched chainization and the destruction of aromatic functional groups are observed to have increased.The low-temperature oxidation markedly enhances the bioavailability of coal,which is the primary driver of the observed increase in methane production.In addition,the alteration of coal structure contributes to a notable shift in microbial community structure.The abundance of Macellibacteroides exhibites a pro-nounced increase,rising from 1.08%in origiual coal to 31.97%,49.18%,and 58.84%for LOC,MOC and HOC,respectively.The abundance of Methanobacterium increases significantly after low-temperature oxidation,indicating a synergistic methanogenesis with Macellibacteroides.Additionally,the results suggest that low-temperature oxidation may tend more to a hydrotrophic methanogenic pathway.At the end of gas production process,the accumulation of acidic organic matter in oxidized coal degradation solution exhibits an increase of 30.99%to 45.25%compared with that in original coal.This phenomenon promotes the esterification reaction,resulting in an elevation of esters in post-reaction percentage.[Conclusions]The study demonstrates that low-temperature oxidized coal in goaf is more conducive to biodegradation,which provides a theoretical basis for the implementation of coal-bed methane bioengineering in the goaf.关键词
煤层气生物工程/低温氧化煤/官能团/菌群结构Key words
coalbed methane bioengineering/low-temperature oxidized coal/functional groups/microbial community structure分类
资源环境引用本文复制引用
高龙珍,米申昱,刘英超,尹贻凡,郭红光..低温氧化煤生物降解产甲烷机理研究[J].太原理工大学学报,2025,56(4):656-664,9.基金项目
国家自然科学基金(U1810103,51404163) （U1810103,51404163）
山西省重点研发计划(国际合作项目,201903D421088) （国际合作项目,201903D421088）
