西安交通大学学报（医学版）2025，Vol.46Issue(4)：599-605,7.DOI:10.7652/jdyxb202504008
毛冬青皂苷E通过HIF-2α调控细胞焦亡对心肌梗死后心肌损伤的影响
Effect of erioside E on myocardial injury after myocardial infarction by regulating pyroptosis through HIF-2α
摘要
Abstract
Objective To investigate the effect of erioside E on myocardial injury after myocardial infarction(MI)by regulating hypoxia-inducible factor-2α(HIF-2α)-mediated pyroptosis of cardiomyocytes and its possible mechanism.Methods Thirty rats were randomly divided into sham-operation group,MI group,and erioside E group,with 10 rats in each group.Cardiomyocytes H9C2 were cultured and divided into control group,hypoxia group,erioside E group and HIF-2α overexpression group.Masson staining was used to observe myocardial fibrosis and infarct size.The protein expressions of HIF-2α,NLRP3,Caspase-1 and GSDMD were detected by Western blotting.RT-qPCR was used to detect the mRNA expressions of HIF-2α,NLRP3,Caspase-1 and GSDMD.EdU kit was used to detect the proliferation of cardiomyocytes.The expressions of NLRP3,Caspase-1 and GSDMD proteins in cardiomyocytes were detected by immunofluorescence staining.Results Compared with the sham-operation group,the myocardial fibrosis level and infarct size were significantly increased in the MI group(P＜0.05),and the protein expressions of HIF-2α,pyroptosis-related proteins of NLRP3,Caspase-1 and GSDMD in myocardial tissue were increased(P＜0.05).Compared with the MI group,the level of myocardial fibrosis and infarct size were significantly decreased(P＜0.05),the protein expression of HIF-2α in myocardial tissue was increased,and the protein expressions of NLRP3,Caspase-1 and GSDMD were decreased in the erioside E group(P＜0.05).Compared with the control group,the hypoxia group had a significant reduction in the proliferation of cardiomyocytes and significant increase in the mRNA and protein expressions of HIF-2α,NLRP3,Caspase-1 and GSDMD(P＜0.05).Compared with the hypoxia group,the proliferation ability of cardiomyocytes was significantly increased,and the expressions of HIF-2α,NLRP3,Caspase-1 and GSDMD mRNA in cardiomyocytes were decreased in the erioside E group(P＜0.05),while the protein expression of HIF-2α in cardiomyocytes was increased in the HIF-2αoverexpression group.The protein expressions of NLRP3,Caspase-1 and GSDMD were significantly decreased(P＜0.05).Conclusion Erioside E can improve myocardial injury by upregulating HIF-2α to inhibit myocardial pyroptosis.关键词
心肌梗死(MI)/毛冬青皂苷E/缺氧诱导因子-2α(HIF-2α)/细胞焦亡/缺氧Key words
myocardial infarction(MI)/erioside E/hypoxia-inducible factor-2α(HIF-2α)/pyroptosis/hypoxia分类
医药卫生引用本文复制引用
喻丽,金静,刘芷静,曾曼钰,陈悠优,徐琪..毛冬青皂苷E通过HIF-2α调控细胞焦亡对心肌梗死后心肌损伤的影响[J].西安交通大学学报（医学版）,2025,46(4):599-605,7.基金项目
湖南省卫生健康委科研课题计划项目(No.D202302156127)Supported by Scientific Research Project of Hunan Provincial Health Commission(No.D202302156127) （No.D202302156127）
