西北农业学报2025，Vol.34Issue(8)：1395-1400,6.DOI:10.7606/j.issn.1004-1389.2025.08.003
鸭精液冷冻程序优化及抗氧化效果研究
Optimization of Cryopreservation Protocols and Antioxidant Efficacy in Duck Semen
摘要
Abstract
As a pivotal technique for genetic resource preservation and artificial breeding in waterfowl,sperm cryopreservation faces challenges due to the unique morphological and biochemical characteris-tics of avian spermatozoa,which render them highly susceptible to oxidative stress-induced motility decline during freezing.Using the Jinding duck as a model,this study systematically evaluated theef-fects of four distinct cryopreservation protocols(P1-P4)on sperm quality and oxidative stress re-sponses.A programmable freezer was used to execute multi-stage temperature modulation strategies,with sperm parameters quantified via automated sperm analysis and oxidative stress biomarkers as-sessed.The results demonstrated that Protocol P4-featuring an initial slow cooling phase(7℃/min to-35 ℃)followed by rapid freezing(60 ℃/min to-140 ℃)—achieved superior post-thaw out-comes:sperm viability reached 71.41％,mortality decreased to 28.59％,and progressive motility maintained at 46.99％,all significantly better than those in other protocols.In contrast,Protocol P2 exhibited the poorest performance(viability:34.28％;mortality:65.72％;motility:22.69％).An-tioxidant profiling revealed that P4-maintained superoxide dismutase(SOD)activity at levels compa-rable to fresh semen,while catalase(CAT)activity was significantly higher than that in P2.Notably,malondialdehyde(MDA)levels in P4 showed no significant elevation,whereas P2 demonstrated marked reductions in SOD,CAT,and total antioxidant capacity(T-AOC),along with significant MDA accumulation.Mechanistically,the biphasic cooling strategy of P4 optimized the balance between cellu-lar dehydration and ice crystal inhibition while minimizing the duration of hyperosmotic exposure,thereby effectively reducing reactive oxygen species(ROS)generation and oxidative damage.Con-versely,P2's multi-stage gradual cooling prolonged hypothermic stress,exacerbating mitochondrial dysfunction and oxidative stress amplification.This study demonstrates that cryopreservation proto-cols directly affect duck sperm quality by modulating oxidative stress,providing theoretical support for the standardization of avian semen cryopreservation technologies in genetic conservation programs.关键词
金定鸭/精液冷冻程序/精子质量/氧化应激/脂质过氧化Key words
Jinding duck/Semen cryopreservation protocol/Sperm quality/Oxidative stress/Lipid peroxidation引用本文复制引用
顾昊天,王逸飞,李慧芳,朱春红,王志成,刘宏祥,宋卫涛,陶志云,曾涛,耿明阳,章双杰,徐文娟..鸭精液冷冻程序优化及抗氧化效果研究[J].西北农业学报,2025,34(8):1395-1400,6.基金项目
国家重点研发计划(2022YFD1300100) （2022YFD1300100）
江苏省种业振兴"揭榜挂帅"项目(JBGS(2021)[030]) （JBGS(2021）
扬州市自然科学基金(YZ2023167) （YZ2023167）
扬州市现代农业项目(YZ2022050) （YZ2022050）
"天池英才"引进计划. National Key R&D Program of China(No.2022YFD1300100) （No.2022YFD1300100）
Project for the Revi-talization of the Seed Industry in Jiangsu Province(No.JBGS(2021)[030]) （No.JBGS(2021）
Yangzhou Natural Sci-ence Foundation(No.YZ2023167) （No.YZ2023167）
Yangzhou Modern Agriculture Development Program(No.YZ2022050) （No.YZ2022050）
"Tianchi Elite Talent"Recruitment Fund. （）
