现代医药卫生2025，Vol.41Issue(7)：1537-1544,8.DOI:10.3969/j.issn.1009-5519.2025.07.001
ccRCC中ATF5表达与舒尼替尼疗效及干细胞特性的相关性分析
Correlation analysis of ATF5 expression with sunitinib efficacy and stem cell characteristics in ccRCC
摘要
Abstract
Objective To explore the difference in the expression levels of activated transcription factor 5(ATF5)in clear cell renal cell carcinoma(ccRCC)and its correlation with the efficacy of targeted therapy drug sunitinib,as well as its correlation with stem cell characteristics.Methods Using ccRCC sample data from the Cancer Genome Atlas(TCGA)database,the samples were divided into a targeted therapy group(in-cluding the sunitinib treatment group)and a non-targeted therapy group.Then R software was used to process and analyze the data,including expression difference analysis,ROC curve analysis,Kaplan-Meier survival anal-ysis,Spearman correlation analysis,and gene set enrichment analysis(GSEA).Results The expression level of ATF5 was significantly elevated in renal cancer patients in the targeted therapy group(P<0.001).ROC curve analysis showed that ATF5 could effectively distinguish between renal cancer tissues in the targeted therapy group and the non-targeted therapy group(AUC=0.714).In the sunitinib treatment group,ATF5 expression levels were also significantly elevated(P<0.001).ROC analysis showed that ATF5 could more accurately distinguish between sunitinib treatment group and non-targeted therapy group renal cancer tissues(AUC=0.745).Kaplan-Meier survival analysis showed that high ATF5 expression was associated with lower overall survival in renal cancer patients receiving sunitinib treatment(P=0.039).Spearman correlation analy-sis showed that ATF5 expression in renal cancer was positively correlated with the expression levels of stem cell-related genes CD44,CXCR4,MYC,and SOX2(P<0.05).GSEA revealed that ATF5 high expression was significantly associated with stem cell characteristics and activated key stem cell characteristic signaling path-ways,including Wnt,Hedgehog,and Cell Cycle(P<0.05).In the tissues of renal cell carcinoma patients trea-ted with sunitinib,the expression levels of stem cell characteristic-related genes CD44 and CXCR4 were signif-icantly increased(P<0.05).Conclusion The high expression of ATF5 is associated with the resistance of ccRCC to targeted therapy,and may be an important biomarker for predicting the efficacy of sunitinib.The ex-pression of ATF5 is closely related to the characteristics of renal cancer stem cells,which may be one of the mechanisms by which it influences the efficacy of sunitinib.关键词
激活转录因子5/肾透明细胞癌/舒尼替尼/靶向治疗/干细胞特性Key words
activated transcription factor 5/clear cell renal cell carcinoma/Sunitinib/Targeted therapy/Stem cell characteristics分类
医药卫生引用本文复制引用
植曦,何烨..ccRCC中ATF5表达与舒尼替尼疗效及干细胞特性的相关性分析[J].现代医药卫生,2025,41(7):1537-1544,8.基金项目
广东省医学科学技术研究基金项目(B2023226). （B2023226）
