现代医药卫生2025，Vol.41Issue(7)：1545-1549,5.DOI:10.3969/j.issn.1009-5519.2025.07.002
ANK2对卵巢癌OVCAR5细胞干性的体外作用研究
Effect of ANK2 on the stemness of ovarian cancer OVCAR5 cells in vitro
徐嘉曼 1承欧梅1
作者信息
- 1. 重庆医科大学附属第一医院神经内科,重庆 400016
- 折叠
摘要
Abstract
Objective To investigate the effect of ANK2 on the stemness of ovarian cancer OVCAR5 cells in vitro.Methods OVCAR5 cells were infected by ANK2-overexpressing and empty vector control lenti-virus to establish an ANK2-overexpressing model.The cells were divided into the ANK2-overexpressing and empty vector control groups.The expression levels of CD133,SOX2,OCT4 and NANOG in each group were detected by real-time fluorescent quantitative polymerase chain reaction and Western blotting.The frequency of microsphere formation in each group was detected by limiting dilution method in vitro.Results Compared with the empty vector control group,the microspherogenic ability of ANK2-overexpressing group OVCAR5 cells,as well as the mRNA and protein expression levels of CD133,SOX2,OCT4,and NANOG,were signifi-cantly enhanced,and the microspherogenic frequency was significantly increased.The differences were statisti-cally significant(P<0.05).Conclusion ANK2 has an in vitro promoting effect on the dryness of OVCAR5 cells.关键词
ANK2/卵巢肿瘤/OVCAR5细胞/干性/体外作用Key words
ANK2/Ovarian neoplasms/OVCAR5 cells/Dry/In vitro effect分类
医药卫生引用本文复制引用
徐嘉曼,承欧梅..ANK2对卵巢癌OVCAR5细胞干性的体外作用研究[J].现代医药卫生,2025,41(7):1545-1549,5.
