现代医药卫生2025，Vol.41Issue(7)：1575-1577,1583,4.DOI:10.3969/j.issn.1009-5519.2025.07.008
人类精子不同冷冻保存方法的效果研究
Effects of different freezing methods on cryopreservation of human sperm
摘要
Abstract
Objective To observe and analyze the preservation and recovery effects of human sperm un-der different freezing methods,so as to provide help for the assisted treatment of obstructive azoospermia in-fertility patients with ICSI technology.Methods A total of 91 patients in the Jiayin Hospital from January to December 31 were selected,including 40 normal semen samples and 51 semen samples obtained by testicular puncture.Each portion of semen was divided into three equal parts.Group Ⅰ was frozen using ordinary cryo-tubes,Group Ⅱ was frozen using 0.25 mL straw tubes,and Group Ⅲ was frozen using ultra-micro straw tubes.Analysis of variance was used for comparisons among multiple groups,the least significant difference method(LSD method)was used for pairwise comparisons,and paired t-tests were used before and after freez-ing.Results Compared with before freezing(72.7%),the total motility ratio of sperm after cryopreservation using three different carriers(Group Ⅰ:35.68%,Group Ⅱ:38.03%,Group Ⅲ:40.13%)was significantly re-duced(P<0.05).In normal semen samples,the total sperm motility and proportion of forward moving sperm were higher in group Ⅲ after freezing and thawing.The recovery rate of group Ⅲ(56.07%)was higher than that of group Ⅲ(49.05%),and the difference was significant(P<0.05);In the semen samples obtained by testicular puncture,the proportion of active sperm after frozen resuscitation in Group Ⅱ and Group Ⅲ(22.59%,24.47%)was higher than that in Group Ⅰ(17.77%),and the resuscitation rates in Group Ⅱ and Group Ⅲ(49.72%,52.96%)were also higher than those in Group Ⅰ(34.73%),with statistical significance(P<0.05).The resuscitation rates among the three carriers were ranked from high to low as Group Ⅲ>Group Ⅱ>Group I.Conclusion Among the three freezing methods,ultra-micro straws are most conducive to the cryopreservation of human sperm,showing the best preservation effect for both normal source sperm and sperm obtained through testicular incision and microscopic sperm retrieval surgery.关键词
冷冻方法/精子冷冻保存/冷冻复苏Key words
Freezing method/Sperm cryopreservation/Cryop reservation分类
医药卫生引用本文复制引用
梁齐,黄卫东,孙健,左亚军,邵秀花..人类精子不同冷冻保存方法的效果研究[J].现代医药卫生,2025,41(7):1575-1577,1583,4.基金项目
新疆维吾尔自治区卫生健康委员会青年医学科技人才专项科研项目(WJWY-201925). （WJWY-201925）
