现代中西医结合杂志2025，Vol.34Issue(11)：1488-1493,6.DOI:10.3969/j.issn.1008-8849.2025.11.005
丹皮酚调控HIF-1α/PI3K/Akt通路对子宫内膜癌Ishikawa细胞的影响
Paeonol inhibits endometrial carcinoma Ishikawa cells via HIF-1α/PI3K/Akt pathway
摘要
Abstract
Objective It is to explore the inhibitory effects of paeonol(PAE)on proliferation,migration and invasion of human endometrial carcinoma(EC)Ishikawa cells and its underlying mechanisms via hypoxia-inducible factor-1α(HIF-1α)/phosphatidylinositide 3-kinases(PI3K)/protein kinase B(Akt)signaling pathway.Methods Ishikawa cells were cul-tured in vitro,and the optimal concentration and intervention time of PAE were screened by CCK-8 assay.The cells were divided into 3 groups:the control group was cultured in medium containing 0.1%DMSO,the PAE group was cultured in medium containing 50 μmol/L PAE,and the cisplatin group was cultured in medium containing 10 μmol/L cisplatin.After 24 hours of culture,the cell viability was detected by CCK-8 method,and the cell survival rate was calculated;the cytotox-icity was detected by lactate dehydrogenase(LDH)release assays,and the LDH release rate was calculated;the cell mi-gration and invasion abilities were assessed by scratch test and Transwell test,and the cell migration rates and invasion rates were calculated;the mRNA expressions of HIF-1α,PI3K,Akt,and vimentin in the cells were detected by qPCR.Results The cell survival rate,cell migration rate,cell invasion rate,and relative mRNA expressions of HIF-1α,PI3K,Akt and vimentin in the cells of the PAE group and cisplatin group were significantly lower than those in the control group(all P<0.05),but there were no statistically significant differences in the above indicators between the PAE group and cisplatin group(all P>0.05).The LDH release rates of the PAE group and cisplatin group were significantly higher than that of the control group(both P<0.05),and the LDH release rate of the PAE group was significantly lower than that of the cisplatin group(P<0.05).Conclusion PAE can inhibit the proliferation,migration and invasion of Ishikawa cells via inhibiting HIF-1α/PI3K/Akt signaling pathway and down-regulating expression of Vimentin.Its effect is comparable to that of cisplatin,but with lower cytotoxicity.关键词
子宫内膜癌/丹皮酚/HIF-1α/PI3K/Akt信号通路/增殖/迁移/侵袭Key words
endometrial cancer/paeonol/HIF-1α/PI3K/Akt signal pathway/proliferation/migration/invasion分类
医药卫生引用本文复制引用
肖春辉,李清雪,姜璇,朱斯斯,武莹,成浩媛,谷素彦,冯紫霞,张英辉..丹皮酚调控HIF-1α/PI3K/Akt通路对子宫内膜癌Ishikawa细胞的影响[J].现代中西医结合杂志,2025,34(11):1488-1493,6.基金项目
石家庄市科学技术研究与发展计划项目(211200603) （211200603）
