基于近红外光谱技术建立橡胶草根部菊糖和总糖含量快速检测方法
A rapid determination method for inulin and total sugar content in araxacum kok-saghyz Rodin based on near-infrared spectroscopy
摘要
Abstract
[Objective]This study aims to develop a green,low-cost,rapid detection technique for inu-lin and total sugar content in the roots of Taraxacum kok-saghyz Rodin(TKS)using near-infrared(NIR)spectroscopy,thereby advancing research in cultivation and breeding of TKS.[Methods]This study involved 103 dry root powder samples of TKS(Rubber Grass).Spectra were collected using the FOSS NIRSTM DS2500F SR near-infrared spectrometer(wavelength range 850-2500 nm)by Foss Company.Enzyme la-beling method was employed to measure the total sugar and reducing sugar content in the root samples and the inulin content was calculated by subtracting the reducing sugar content from the total sugar content.The SPXY algorithm was utilized to divide the samples into training and validation sets.Pretreatments on the raw spectral data included Moving Window Smoothing(MWS),Standard Normal Variate(SNV),Multiplicative Scatter Correction(MSC),and First Derivative(FD).Competitive Adaptive Reweighted Sampling(CARS)was ap-plied for wavelength selection,followed by establishing Partial Least Squares(PLS)prediction models for inu-lin and total sugar content in TKS roots.[Results]The content range of total sugar,reducing sugar,and inu-lin in the sample set was 9.79％-51.85％,3.11％-8.98％,and 6.41％-45.35％,respectively,with variation coefficients of 34.18％,32.61％,and 39.46％.The samples were divided into calibration and vali-dation sets at a ratio of 4∶1 using the SPXY algorithm,with each set containing 82 and 21 samples for both to-tal sugar and inulin.The optimal PLS prediction model for inulin content utilized MWS-SNV-CARS prepro-cessing,achieving a validation set correlation coefficient(Rv)of 0.942,a root mean square error(RMSEv)of 2.515,and a relative performance deviation(RPDv)of 2.977.For total sugar,the best PLS model also employed MWS-SNV-CARS preprocessing,reaching an Rv of 0.949,RMSEv of 2.490,and RPDv of 3.175.Both models exhibited RPDv values exceeding 2.5,indicating good performance.[Conclusion]NIR spectroscopy proves to be an effective method for the green,low-cost,and rapid quantitative determination of inulin and total sugar contents in the roots of TKS.关键词
橡胶草/近红外光谱/菊糖/总糖/快速检测/PLSKey words
Taraxacum kok-saghyz Rodin/near-infrared spectroscopy/inulin/total sugar/rapid de-tection/partial least squares分类
农业科技引用本文复制引用
陈润峰,高强,严青青,徐麟,吐汗姑丽·托合提,张龑,郑立鹏,任海龙,聂秋海..基于近红外光谱技术建立橡胶草根部菊糖和总糖含量快速检测方法[J].新疆农业科学,2025,62(5):1102-1110,9.基金项目
"天山英才"培养计划项目"橡胶草种质资源挖掘与创新利用研究"(2023TSYCLJ0010) （2023TSYCLJ0010）
新疆农业科学院青年科技骨干创新能力培养项目"栽培条件下不同生态型橡胶草橡胶积累规律研究"(xjnkq-2023016) （xjnkq-2023016）
新疆维吾尔自治区自然科学基金青年基金"基于近红外光谱技术建立蒲公英橡胶草橡胶检测模型的研究"(2022D01B33) （2022D01B33）
新疆农业科学院科技创新稳定支持项目"耐盐橡胶草种质资源筛选及优异基因挖掘"(xjnkywdzc-2023001-28) （xjnkywdzc-2023001-28）
新疆农业科学院自主培育项目"新疆农作物种质资源优异特性挖掘与利用"(nkyzzkj-008) （nkyzzkj-008）
新疆农业科学院自主培育项目"农作物种质资源繁殖与利用团队"(nkyzztd-004) Tianshan Talent Cultivation Program:"Exploration and Innovative Utilization of Germplasm Resources in Taraxacum kok-saghyz Rodin"(2023TSYCLJ0010) （nkyzztd-004）
The Young Scientific and Technological Backbone Innovation Capability Cultivation Project of Xinjiang Academy of Agricultural Sciences:"Study on the Accumulation Regularity of Rubber in Different Ecotypes of Taraxacum kok-saghyz Rodin under Cultivation Conditions"(xjnkq-2023016) （xjnkq-2023016）
the Natural Science Foundation Youth Fund of Xinjiang Uygur Au-tonomous Region:"Establishment of a Rubber Detection Model for Taraxacum kok-saghyz Rodin based on Near-Infrared Spec-troscopy"(2022D01B33) （2022D01B33）
The Science and Technology Innovation Stability Support Project of Xinjiang Academy of Agricultural Sci-ences:"Screening of Salt-Tolerant Germplasm Resources in Taraxacum kok-saghyz Rodin and Excavation of Superior Genes"(xjnkywdzc-2023001-28) （xjnkywdzc-2023001-28）
The Independent Cultivation Project of Xinjiang Academy of Agricultural Sciences:"Exploration and Utilization of Superior Traits in Xinjiang Crop Germplasm Resources"(nkyzzkj-008) （nkyzzkj-008）
The Independent Cultivation Project of Xin-jiang Academy of Agricultural Sciences"Crop Germplasm Resources Propagation and Utilization Team"(nkyzztd-004) （nkyzztd-004）
