新疆农业科学2025，Vol.62Issue(5)：1121-1130,10.DOI:10.6048/j.issn.1001-4330.2025.05.009
不同马铃薯耐盐性转录组比较及耐盐基因的挖掘
Comparative transcriptome analysis between two potato varieties with different salt-tolerance and further identification of potato salt-tolerance genes
摘要
Abstract
[Objective]To discover candidate genes related to salt tolerance in potatoes and provide theo-retical basis for the identification and evaluation of salt tolerance in potatoes and the exploration of salt toler-ance genes.[Methods]The salt tolerant variety Jinshu 16 and salt sensitive variety Jizhangshu 12 were used as test materials.Salt stress treatments were carried out using 0％,0.5％,1％,and 1.5％NaCl solutions.On the 8th day,plant phenotypes and physiological and biochemical indicators were analyzed.Subsequently,a 1％salt concentration was selected for treatment,and transcriptome sequencing and bioinformatics analysis were performed on the two varieties.[Results]Compared to Jizhangshu 12,Jinshu 16 had lower malondialde-hyde content under salt stress conditions.Transcriptome data showed significant differences in plant hormone signaling pathways and flavonoid metabolism pathways between the two varieties.[Conclusion]Based on the above results,several differentially expressed genes related to plant hormone signal transduction and flavonoid metabolism are screened,which provides genetic resources for improving salt tolerance in potatoes.关键词
马铃薯/盐胁迫/转录组测序/植物激素信号途径/类黄酮代谢途径Key words
potato/salt stress/transcriptome sequencing/plant hormone signaling pathways/flavonoids metabolic pathways分类
农业科技引用本文复制引用
王亚玲,江应红,孙慧,刘易..不同马铃薯耐盐性转录组比较及耐盐基因的挖掘[J].新疆农业科学,2025,62(5):1121-1130,10.基金项目
新疆维吾尔自治区自然科学青年基金项目"基于转录组测序马铃薯耐盐基因的挖掘"(2021D01B69) （2021D01B69）
新疆维吾尔自治区重点研发任务专项"新疆马铃薯产业提质增效关键技术研究"(2022B02044) （2022B02044）
国家马铃薯产业技术体系-乌鲁木齐综合试验站(CARS-09-ES36) Natural Science Foundation of Xinjiang Uyghur Autonomous Region"Comparative transcriptome analysis between two potato varieties with different salt-tolerance and further identification of potato salt-tolerance genes"(2021D01B69) （CARS-09-ES36）
the Project of the Key R & D Special Task of Xinjiang Uygur Autonomous Region"Research on Key Technologies for Quality Improvement and Efficiency En-hancement in Potato Industry"(2022B02044) （2022B02044）
China Agriculture Research System—Urumqi Comprehensive Experimental Station(CARS-09-ES36) （CARS-09-ES36）
