畜牧兽医学报2025，Vol.56Issue(7)：3399-3407,9.DOI:10.11843/j.issn.0366-6964.2025.07.032
猪源致病性格氏乳球菌的分离鉴定及毒力基因分析
Isolation,Identification and Virulence Gene Analysis of Porcine Pathogenic Lactococcus garvieae
摘要
Abstract
This study aimed to explore the biological characteristics,drug sensitivity and pathogenicity of Lactococcus garvieae from pig.Suspicious bacteria were isolated from the liver of diseased pig,and then were identified by Gram stained microscopy,matrix assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS),16S rRNA gene sequence analysis and sequence analysis.The drug resistance and pathogenicity of isolated strains were explored through drug sensitivity testing and mice infection testing.Related virulence of isolated strain genes were amplified by PCR.Results were as follows:A positive strain of a-hemolytic was isolated from the liver of a diseased pig.MALDI-TOF MS,PCR amplification of 16S rRNA gene and phylogenetic tree analysis results showed that the isolated strain was identified as Lactococcus garvieae.The infection test of mice showed that the isolated strain had strong lethality.It can cause swelling of the liver and spleen,severe congestion of the intestines,abdominal cavity,and chest cavity in mice.The drug sensitivity test results showed that the isolated strains were resistant to β-lactam drugs such as penicillin G and ampicillin,as well as erythromycin and lincomycin,and sensitive to other antibiotics.The results of virulence gene testing showed that the isolated strain contains adhesion related genes adhC1,psaA,eno,lp1,hemolysis related genes hly1,hly2,hly3,capsule gene cluster formation related genes cgcC,polysaccharide related genes epsA,epsB,epsC,epsD,epsL,and other virulence genes nadho,sod,pgm,chp.The Lactococcus garvieae was isolated in this experiment has strong pathogenicity,and its potential risks cannot be ignored as it belongs to zoonotic pathogens.This study provides data reference for the prevention and treatment of clinical infections caused by Lactococcus garvieae.关键词
格氏乳球菌/猪/16S rRNA/药敏试验/毒力基因Key words
Lactococcus garvieae/pig/16S rRNA/drug susceptibility test/virulence gene分类
农业科技引用本文复制引用
舒静超,张菡,彭志锋,乔宏兴..猪源致病性格氏乳球菌的分离鉴定及毒力基因分析[J].畜牧兽医学报,2025,56(7):3399-3407,9.基金项目
河南省现代农业生猪体系中药替抗岗位专家项目(HARS-22-12-G2) （HARS-22-12-G2）
河南省重点研发专项项目(241111113400) （241111113400）
河南牧业经济学院校重点学科项目(XJXK202202) （XJXK202202）
