畜牧与兽医2025，Vol.57Issue(8)：78-84,7.
猪细小病毒2型VP2蛋白的原核表达及其多克隆抗体的制备
Prokaryotic expression of VP2 protein of porcine parvovirus 2 and preparation of its polyclonal antibody
摘要
Abstract
In this study,polyclonal antibodies to PPV VP2 were designed,prepared,and validated with porcine parvovirus(PPV)and eukaryotic plasmid pcDNA3.1(+)-VP2-Myc.PPV VP2 was then cloned into the prokaryotic expression vector pCold-TF,and VP2 pro-tein was induced by 1 mmol/L IPTG.Next,the purified VP2 protein was immunized against New Zealand white rabbits,and polyclonal anti-bodies were prepared and subjected to ELISA,Western blot;and IFA was used to verify their reactivity.The results of ELISA showed that the titer of the polyclonal antibody was as high as 1∶128 000.The results of Western blot and IFA showed that the antibody could specifical-ly recognize and bind to PPV,indicating that the recombinant VP2 protein had good immunogenicity and reactogenicity.This study is condu-cive to the establishment of a VP2 serological detection method and the functional study of VP2 protein.关键词
猪细小病毒/VP2蛋白/多克隆抗体/原核表达Key words
porcine parvovirus/VP2 protein/polyclonal antibodies/prokaryotic expression分类
农业科技引用本文复制引用
帅文娜,李兆龙,高飞,李佳乐,郭子强,罗梦,李丽薇,周艳君,姜一峰,童武,童光志..猪细小病毒2型VP2蛋白的原核表达及其多克隆抗体的制备[J].畜牧与兽医,2025,57(8):78-84,7.基金项目
国家重点研发计划项目(2022YFD1800800,2021YFD1801401) （2022YFD1800800,2021YFD1801401）
上海市自然科学基金项目(21ZR1476900) （21ZR1476900）
国家自然科学基金项目(32373050,32072861) （32373050,32072861）
中国农业科学院科技创新工程项目(CAAS-CSLPDCP-202402) （CAAS-CSLPDCP-202402）
上海市兽医生物技术重点实验室开放基金项目(BD1500010) （BD1500010）
