畜牧与兽医2025，Vol.57Issue(8)：85-94,10.
猪肺炎支原体感染3D4/21细胞的基因表达谱分析
Gene expression profile of 3D4/21 cells infected with Mycoplasma hyopneumoniae
摘要
Abstract
The study was to investigate the changes in ROS secretion and related gene differential expression following Mycoplasma hyo-pneumoniae(Mhp)infection of 3D4/21 cells,and to analyze the functions and potential infection mechanisms of these differentially ex-pressed genes by selecting appropriate time points.High-throughput sequencing technology was used to analyze gene expression in Mhp-in-fected cells,and functional enrichment analysis was performed on the differentially expressed genes(DEGs)using GO and KEGG.The results showed that there were 1 940 differentially expressed genes between Mhp-infected cells and blank control cells,with 1 070 upregulat-ed and 871 downregulated.The GO functional analysis revealed that the impact of Mhp on 3D4/21 cells primarily focused on cellular proces-ses,metabolic processes,and biological regulation.The KEGG enrichment analysis indicated that the differentially expressed genes were mainly enriched in pathways related to cancer development,redox reactions,chemical carcinogenesis,MAPK signaling,and PI3K-Akt sig-naling.This study provided new insights into the molecular mechanisms of Mhp infection and laid a foundation for the development of targeted drugs for the prevention and treatment of Mycoplasmal pneumonia in swine.关键词
猪肺炎支原体/转录组测序/基因差异表达谱Key words
Mycoplasma hyopneumoniae/transcriptome sequencing/gene differential expression profiling分类
农业科技引用本文复制引用
卢冰霞,秦毅斌,段群棚,许艺兰,全琛宇,陈婷婷,徐悦,吴先华,陈忠伟,周颖,许心婷,杨讯业,林昌华,赵硕,程珂,张胜斌,覃国喜..猪肺炎支原体感染3D4/21细胞的基因表达谱分析[J].畜牧与兽医,2025,57(8):85-94,10.基金项目
国家现代农业产业技术体系广西创新团队建设专项(nycytxgxcxtd-2023-15-02) （nycytxgxcxtd-2023-15-02）
南宁市科学研究与技术开发计划项目(20232039) （20232039）
广西基本科研业务费专项(桂科专项23-4) （桂科专项23-4）
广西畜牧产业科技先锋队项目(桂农科盟202409-01-3) （桂农科盟202409-01-3）
广西农业科技自筹经费项目(Z2023033,Z202222) （Z2023033,Z202222）
广西创新驱动发展专项资金项目(桂科AA17204057-1) （桂科AA17204057-1）
