西南农业学报2025，Vol.38Issue(4)：769-777,9.DOI:10.16213/j.cnki.scjas.2025.4.010
贵州魔芋软腐病新病原土生拉乌尔菌(Raoultella terrigena)及其杀菌剂室内筛选
Raoultella terrigena,a new native pathogen causing konjac soft rot and its indoor screening of bactericides in Guizhou
摘要
Abstract
[Objective]The species of pathogen causing soft rot of Amorphphallus konjac seed tubers are identified in Weining county,Bijie re-gion,Guizhou province,and the effective bactericides against the pathogen of A.konjac soft rot were screened to provide a theoretical basis for control of A.konjac soft rot.[Method]The pathogenicity of the purified pathogen strains isolated from the diseased A.konjac seed tubers was detected by Koch's postulate and the purified pathogen strains were identified by molecular biology and physiological biochemistry.The indoor toxicity of 10 bactericides including 18％Chunleimycin.copper rosin acid SC,25％Brothermothingonil ME and 0.3％Tetramycin AS,etc.against the pathogen causing A.konjac soft rot was determined by the inhibition zone method to screen the bactericides with a significant anti-bacterial effect.[Result]The physiological and biochemical characteristics of the isolated pathogenic strain Akst2-3 causing soft rot of A.kon-jac seed tubers were consistent with Raoultella sp.and the homology between strain Akst2-3 and R.terrigena JCM 1687 reached 99.79％.The phylogenetic tree revealed that strain Akst2-3 and R.terrigena were located on the same branch.Therefore,strain Akst2-3 could be iden-tified as R.terrigena.The indoor toxicity measurement indicated that five bactericides of 18％Chunleimycin·copper rosin acid SC,80％Ethylicin EC,25％Brothermothingonil ME,0.3％Tetramycin AS and 5％Garlicin ME had the significant inhibitory effect against the patho-gen causing soft rot of A.konjac seed tubers.The EC50 of 18％Chunleimycin·copper rosin acid SC,80％Ethylicin EC,25％Brothermothin-gonil ME,0.3％Tetramycin AS and 5％Garlicin ME was 0.5282,1.3023,1.6882,2.0886 and 13.8071 mg/L respectively.[Conclusion]The R.terrigena is a new pathogen causing soft rot of A.konjac seed tubers.18％Chunleimycin·copper rosin acid SC and 80％Ethylicin EC can be selected as the preferred bactericides for controlling the native R.terrigena causing soft rot of A.konjac seed tubers.25％Bromothalo-nil ME and 0.3％Tetramycin AS can be used as the alternative bactericides in control of A.konjac soft rot.Four bactericides should be alter-nately and mixedly used to prevent the resistance risk of a single bactericide in A.konjac production.关键词
花魔芋/软腐病/土生拉乌尔菌/药剂筛选Key words
Amorphophallus konjac/Soft rot/Raoultella terrigena/Bactericide selection分类
农业科技引用本文复制引用
罗林丽,刘思睿,童安毕,赵兴丽,丁海兵,范士杰..贵州魔芋软腐病新病原土生拉乌尔菌(Raoultella terrigena)及其杀菌剂室内筛选[J].西南农业学报,2025,38(4):769-777,9.基金项目
贵州省科技计划项目(黔科合服企[2021]11号) （黔科合服企[2021]11号）
贵州省农业科学院青年基金项目(黔农科一般基金[2024]20号) （黔农科一般基金[2024]20号）
