西南农业学报2025，Vol.38Issue(5)：901-911,11.DOI:10.16213/j.cnki.scjas.2025.5.003
不同生境烟草番茄斑萎病发生调查及周边毒源植物鉴定
Investigation of occurrence and identification of surrounding virus host plants of tobacco tomato spotted wilt virus in different habitats
摘要
Abstract
[Objective]The study aimed to clarify the occurrence and identify surrounding virus host plants of tomato spotted wilt virus(TSWV)in different habitats of tobacco fields in Chuxiong tobacco-growing area.[Method]From May to July 2024,a systematic investigation was conducted to determine the dispersal distance of the vector thrips and the total number of thrips and the occurrence of TSWV in six differ-ent tobacco fields.The study used RT-PCR technology to detect Tomato spotted wilt virus(TSWV)in tobacco leaves and surrounding crops and weeds in the six habitats,and conducted gene sequencing based on the N protein of TSWV.The study also conducted a MEGA sequence comparison to analyze the genetic relationship of TSWV between tobacco leaves in different habitats and surrounding crops and weeds.[Re-sult]Thrips dispersed from plants outside tobacco fields to tobacco fields within a distance of about 100 m.Among the 6 different habitats in tobacco fields,the highest number of thrips was monitored in the tobacco field near a reservoir with perennial weeds,and the highest TSWV disease index was found in the tobacco field adjacent to vegetables,while the lowest was found in the tobacco field adjacent to maize.The de-tection rate of TSWV was 85.42％of tobacco leaves and surrounding crops and weeds in different habitats,and the phylogenetic tree showed that most crops in the surrounding area of tobacco fields were direct sources of TSWV,such as cucumber,cabbage,bean and citrus.And the surrounding four weeds,such as Alternanthera philoxeroides,Artemisia caruifolia,Ageratum conyzoide and Oenanthe javanica,were virus host plants of TSWV in tobacco.[Conclusion]In order to better prevent and control the occurrence of tobacco tomato spotted wilt virus in tobacco fields,isolated crops such as corn and other grass family can be appropriately planted around tobacco plants,but the appearance of virus host plants shall be avoided.When TSWV host plants are planted around,they shall be more than 100 m away.关键词
烟草/番茄斑萎病毒/周边毒源植物/蓟马Key words
Tobacco/Tomato spotted wilt virus/Peripheral virus host plants/Thrips分类
农业科技引用本文复制引用
胡杨,李佛琳,刘叶青,吴德喜,杨旸,黄文武,李鑫磊,段茂鹤,熊普娟,蔡华勇..不同生境烟草番茄斑萎病发生调查及周边毒源植物鉴定[J].西南农业学报,2025,38(5):901-911,11.基金项目
云南省烟草公司楚雄州公司科技计划一般项目(2023532301cy003) （2023532301cy003）
