西南农业学报2025，Vol.38Issue(5)：964-975,12.DOI:10.16213/j.cnki.scjas.2025.5.009
半夏OFP基因家族的全基因组鉴定及表达模式分析
Genome-wide identification and expression pattern analysis of OFP gene family in Pinellia ternata
摘要
Abstract
[Objective]Ovate family proteins(OFPs)were a class of plant-specific proteins that played a pivotal role in regulating various as-pects of plant growth and development.The tuber of Pinellia ternate,which served as the principal medicinal component,had a substantial im-pact on the yield and quality of the plant.The objective of the study was to explore the structural features of OFP gene family members within P.ternate,and to assess their potential contributions to the tuber formation process.[Method]Based on the published chromosomal-level ge-nome assembly of P.ternate,bioinformatics methods were employed to identify the OFP gene family members in P.ternate.Following the i-dentification,a comprehensive analysis was carried out.This included examining gene structures,the physicochemical properties of the pro-teins,subcellular localization,conserved motifs,domains,tertiary structures,and promoter cis-acting elements.Phylogenetic,collinearity,and gene duplication and loss analysis were also undertaken,followed by an analysis of its expression patterns using transcriptomic data.[Result]A total of 25 PtOFP genes were identified.Analysis of the proteins' physicochemical properties and subcellular localization revealed that Pt-OFPs were unstable hydrophilic proteins primarily localized in the nucleus.Further examination of conserved motifs,structural domains,gene structure and protein tertiary structure indicated that PtOFPs exhibited relative conservation in both sequence and structure,suggesting poten-tial functional redundancy among some members.Phylogenetic,collinearity,and gene duplication and loss analysis revealed that the expansion of the P.ternata OFP gene family members mainly resulted from segmental duplication and tandem duplication,and they were evolutionarily closer to rice.Analysis of promoter cis-acting elements suggested that PtOFPs might be involved in response to biotic and abiotic stresses and regulate the growth and development of P.ternata through hormonal responses.The expression patterns of PtOFPs genes at different develop-mental stages of tubers and under exogenous brassinolide treatment conditions indicated that PtOFPs were very likely to be involved in the regulation of tuber developmental process through the brassinosteroid(BR)pathway,playing a role in maintaining tuber size.In particular,PtOFP9,PtOFP13 and PtOFP16 merited close attention.[Conclusion]The results of the study will contribute to revealing the involvement of PtOFPs in the tuber development process of P.ternata,thereby establishing a theoretical basis for future breeding,enhancing varieties,optimi-zing cultivation practices,and maximizing the utilization of P.ternata germplasm resources.关键词
半夏/OFP基因家族/基因鉴定/表达模式/生物信息学/块茎Key words
Pinellia ternate/OFP gene family/Gene identification/Expression pattern/Bioinformatics/Tuber分类
农业科技引用本文复制引用
朱志炎,周厚英,刘柳,张德清,何勇,田志宏..半夏OFP基因家族的全基因组鉴定及表达模式分析[J].西南农业学报,2025,38(5):964-975,12.基金项目
江苏省高等学校基础科学(自然科学)研究面上项目(24KJD210003) （自然科学）
南通师范高等专科学校青年专项(TSGZ2022Q02) （TSGZ2022Q02）
亚热带农业生物资源保护与利用国家重点实验室开放课题(SKLCUSA-b202305) （SKLCUSA-b202305）
