烟草科技2025，Vol.58Issue(7)：20-27,8.DOI:10.16135/j.issn1002-0861.2024.0877
木质素降解菌枯草芽孢杆菌YY-10的特性表征及工程菌构建
Characterization of lignin-degrading Bacillus subtilis YY-10 and construction of genetically engineered strains
摘要
Abstract
In order to promote effective utilization of tobacco waste and sustainable production of tobacco enzyme additives,Bacillus subtilis YY-10,a nicotine-resistant lignin-degrading bacterium derived from tobacco,was investigated by genome sequencing,exoprotein composition analysis and gene editing techniques to evaluate its feasibility as an enzyme-producing host.The results showed that:1)The YY-10 genome had a circular chromosome encoding 4 252 genes,which contained various active carbohydrate enzymes.The exoproteins of YY-10 were rich in pectate lyase and amylase.2)A pectate lyase expression system was developed and introduced into the YY-10 host,resulting in a genetically engineered strain,YY-10-pelA.The strain achieved expression and secretion of the target enzyme,and exhibited enzyme activity about three times higher than the wild type,and demonstrated the ability to utilize alkaline lignin for growth under nutrient stress.3)Compared with well-established B.subtilis hosts,YY-10 as an enzyme-producing host using tobacco waste for enzyme preparation showed considerable enzyme expression capacity and outstanding advantages in improving the sensory quality of tobacco.关键词
烟草废弃物/木质素降解/枯草芽孢杆菌/酶制剂Key words
Tobacco waste/Lignin degradation/Bacillus subtilis/Enzyme additive分类
轻工纺织引用本文复制引用
程成,姜振锟,丁宁,袁吉锋,范坚强,陈浩锋,陈善义,李菁菁,何伟,龙腾,陈义强,邓小华,周康熙..木质素降解菌枯草芽孢杆菌YY-10的特性表征及工程菌构建[J].烟草科技,2025,58(7):20-27,8.基金项目
福建中烟工业有限责任公司博士后项目"烟草源枯草芽孢杆菌的定向改造与优化"(FJZYKJJH2023BH003). （FJZYKJJH2023BH003）
