烟草科技2025，Vol.58Issue(7)：28-34,43,8.DOI:10.16135/j.issn1002-0861.2024.0588
调制方式对雪茄芯烟氨基酸组成及代谢的影响
Effects of curing methods on amino acid composition and metabolism of cigar filler tobacco
摘要
Abstract
To clarify the influencing mechanism of curing methods on the composition and metabolism of amino acids in cigar filler tobacco,tobacco leaves of cv.Dexue No.4 were cured by two curing methods,air-curing and flue-curing.The differences in contents of amino acids and composition between the leaves cured by the two methods were analyzed,and the non-targeted metabolomics of the cured leaves was studied using liquid chromatography-mass spectrometry(LC-MS/MS).The results showed that the aspartic acid content in air-cured leaves was the highest,accounting for 32.09%of the total free amino acid content,while the proline content was the highest,accounting for 70.84%of the total free amino acid content in flue-cured leaves.A total of 657 differential metabolites were identified at the yellowing stage of tobacco leaves from the two curing methods,of which 573 were higher in air-cured leaves.A total of 106 differential metabolic pathways were enriched and the metabolic pathway most affected by the curing method was amino acid biosynthesis.The contents of oxaloacetate and α-ketoglutarate,which are intermediates of the citric acid cycle in the metabolic synthesis of aspartic acid,glutamic acid,lysine and isoleucine,were higher in air-cured leaves than in that of flue-cured leaves.However,the content of 3-phosphoglyceric acid,which is the glycolytic metabolite of glycolysis in the metabolic synthesis of phenylalanine,cystine and serine,was higher in flue-cured leaves than in air-cured leaves.In conclusion,air-curing may affect the synthesis of amino acids in tobacco leaves by influencing the citric acid cycle pathway,whereas flue-curing may affect the synthesis of amino acids in tobacco leaves by influencing the glycolytic cycle pathway.关键词
雪茄烟叶/调制方式/晾制/烘烤/氨基酸/代谢通路/非靶向代谢组学Key words
Cigar tobacco/Curing method/Air-curing/Flue-curing/Amino acid/Metabolic pathway/Untargeted metabolomics分类
轻工纺织引用本文复制引用
白梦茹,赵园园,王维超,穆文静,蔡宝才,史宏志..调制方式对雪茄芯烟氨基酸组成及代谢的影响[J].烟草科技,2025,58(7):28-34,43,8.基金项目
河南省烟草公司许昌市公司科技项目"许昌禹州晾晒烟开发关键技术研究"(2021411000240097). （2021411000240097）
